LOCUS       sequence-139832-        1055 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Membrane scaffold protein (MSP), helix 4 deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Hagn F, Etzkorn M, Raschle T, Wagner G
  TITLE     Optimized phospholipid bilayer nanodiscs facilitate high-resolution 
            structure determination of membrane proteins.
  JOURNAL   J Am Chem Soc. 2013 Feb 6;135(5):1919-25. doi: 10.1021/ja310901f. 
            Epub 2013 Jan 25.
  PUBMED    23294159
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja310901f";
            journalName: "J Am Chem Soc"; date: "2013-02-6- 6"; volume: "135"; 
            issue: "5"; pages: "1919-25"
COMMENT     Sequence Label: pET28a-MSP1D1delltaH4
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(582..602)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(603..620)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             639..661
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    676..700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(700..719)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(701..719)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(803..822)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa
       61 gcttgtcgac ggagctctta ctgggtgttc agttttttgg tatactcttc cagcgcgctc
      121 agaaagctca ctttaaagct ttccagcacc ggcagcaggc cctgacgcag atcttccagc
      181 gccggtttcg ctttttcgct cagggtgctc agatgttcgg tcgctttcgc atgatattcc
      241 gccagacgcg caccgccgtt ttctttcagc gcttccagac gggccgccag acgctgacgc
      301 agttcatcgc tatacggcgc cagatgggta cgcagcgcat ccacatgcgc acgcgcacga
      361 tcacgcatct cttcgcccag cgggctcagt ttttcctgca gttcatgcag tttctgacgc
      421 gcgccttcct gcagttccgc acgcagcggc tgcactttcg ctttcacctc ttccagatct
      481 ttgctcattt cctgacgcag gccttcggtt tctttttcca ggttatccca aaattcctgg
      541 gtcaccgggc ccagctgttc acgcagtttg ctaaaggtgc tgccctgaaa atacaggttt
      601 tcatgatgat gatgatgatg gctgctgccc atggtatatc tccttcttaa agttaaacaa
      661 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa
      721 tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc
      781 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
      841 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
      901 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
      961 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
     1021 gatcccggac accatcgaat ggcgcaaaac ctttc
//
