LOCUS       sequence-139871-        7391 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses the Sema3G, isoform a protein (truncated at cleavage site 
            P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 
            6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3g.a(S)-Fc-His
FEATURES             Location/Qualifiers
     source          1..7391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3971..3988
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4014..4135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4051..4070)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4105..4124
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4173..4190)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4344..4932)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4424..4443)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5103..5894,5895..5963))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5726..5745
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5964..6068)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6136..6154
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6192..6214)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6216..6236)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6221..6551
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6402..6537
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6464..6483
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6688..6707
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6774..7229)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6911..6932)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7123..7142
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7355..7377
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7369..7386
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7370..7386
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggacccatc
     1621 tgcgtgggcc atctgctgcc tcctggggag ccttctgttc catgtgggca tccccagccc
     1681 tggtcccagc cccagtgtgc ctcgtctgcg gctctcctac agagatctcc tgtctaccaa
     1741 ccgctctgcc atctttctgg gcccacgggg ttccctagac ctccaagtca tgtacctgga
     1801 tgagtatcga gatcgcctct ttctgggaag ccgagatgcc ctttactctc tgagactgga
     1861 tcaggcatgg ccagaccccc gggaggtctt gtggctgcca cagccaggac agaaggtgga
     1921 atgtgtccgc aagggaaaag accctttgac ggagtgtgcc aacttcgtgc gggtcttaca
     1981 accccataac cggacccatc tgctggcctg tggcacagga gccttccaac ccatctgcac
     2041 cttcatcaca gtggggcatc gaggggagca tgtgctccgc ttggatgcta gcagcgtcga
     2101 gaatggaagg gggcgttgcc cacatgagcc cagccgaccc ttcgccagca cctttgtagg
     2161 tggggagcta tacacaggtc tcactgccga tttcctggga cgtgaggcca tgattttccg
     2221 gagtgggggt ccccgaccag ccctgcgttc tgactctgac cagagcctcc tacatgaacc
     2281 ccggtttgtg atggctgctc ggatcccaga taactctgac cgggacgacg acaaggtgta
     2341 cttcttcttc tccgagactg tcccgtcacc agatggtggc ccaggtcatg tcaccatcag
     2401 ccgagtgggc cgcgtctgtg tgaatgatgc tggtggccag cgggtgctgg tgaacaaatg
     2461 gagcactttc ctcaaggccc ggctggtgtg ctctgtgcct ggcccgggtg gggctgagac
     2521 tcactttgac cagctagagg atgtgttcct gctgtggccc aaggccggga agagcctcga
     2581 ggtgtatgcg ctgttcagca ctgtcagtgc tgtgttccag ggctttgctg tctgtgtgta
     2641 ccacatggta gacatctggg aggtcttcaa tgggcccttt gcccaccgag atggccctca
     2701 gcatcagtgg ggaccctatg ggggcaaggt gcccttccct cgccccggtg tgtgtcctag
     2761 caagatgacc gcacagccag gccgaccctt tggcagcacc aaggactacc cagacgaggt
     2821 gttgcagttt gtccgagacc acccactcat gttccagcct gtgaggcctc ggcgtggccg
     2881 ccctgtcctg gtcaaaactc acttggctca gcgactgcgc cagattgtgg tggatcgagt
     2941 ggaggctgag gatgggacct atgatgtcat cttcctaggg actgattcgg gttctgtgct
     3001 caaagtcatt gccctccagg gtggtggctt gactgaacct gaagaggtgg ttttggagga
     3061 gctccaggtg tttaaggtgc caacgcccat cactgagatg gagatctctg tcaaaaggca
     3121 aacgctgtat gtgggctctc cactgggtgt ggcccggctg cagctgcacc agtgtgagac
     3181 ttacggcagt gcctgtgctg agtgctgcct ggcccgggac ccatactgtg cttgggatgg
     3241 cactgcctgt gcccgctacc gccccagctc tggcggcgcg cctgacaaaa ctcacacatg
     3301 cccaccgtgc ccagcacctg aactcctggg gggaccgtca gtcttcctct tccccccaaa
     3361 acccaaggac accctcatga tctcccggac ccctgaggtc acatgcgtgg tggtggacgt
     3421 gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg gacggcgtgg aggtgcataa
     3481 tgccaagaca aagccgcggg aggagcagta caacagcacg taccgtgtgg tcagcgtcct
     3541 caccgtcctg caccaggact ggctgaatgg caaggagtac aagtgcaagg tctccaacaa
     3601 agccctccca gcccccatcg agaaaaccat ctccaaagcc aaagggcagc cccgagaacc
     3661 acaggtgtat accctgcccc catcccggga tgagctgacc aagaaccagg tcagcctgac
     3721 ctgcctggtc aaaggcttct atcccagcga catcgccgtg gagtgggaga gcaatgggca
     3781 gccggagaac aactacaaga ccacgcctcc cgtgctggac tccgacggct ccttcttcct
     3841 ctacagcaag ctcaccgtgg acaagagcag gtggcagcag gggaacgtct tctcatgctc
     3901 cgtgatgcat gaggctctgc acaaccacta cacgcagaag agcctctccc tgtctccggg
     3961 taaaggcggc catcatcacc atcaccatta atctagagat cttattaaag cagaacttgt
     4021 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4081 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4141 tctggtcgac tctagctaga ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4201 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4261 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4321 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4381 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4441 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4501 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4561 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4621 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4681 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4741 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4801 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4861 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4921 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4981 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5041 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5101 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5161 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5221 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5281 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5341 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5401 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5461 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5521 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5581 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5641 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5701 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5761 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5821 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5881 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5941 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6001 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6061 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     6121 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     6181 gacggtgaaa acctctgaca catgcagctc ccggaggaat gtgtgtcagt tagggtgtgg
     6241 aaagtcccca ggctccccag gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6301 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6361 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6421 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6481 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6541 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cgtcccggag acggtcacag
     6601 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     6661 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     6721 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
     6781 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     6841 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     6901 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     6961 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     7021 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     7081 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     7141 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     7201 cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg
     7261 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     7321 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     7381 gccagtgaat t
//
