LOCUS       sequence-140094-        1056 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  UAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNA 
            with 464 amino acid change of Tyrosine to Alanine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Nakagawa T, Pellegrino M, Sato K, Vosshall LB, Touhara K
  TITLE     Amino acid residues contributing to function of the heteromeric 
            insect olfactory receptor complex.
  JOURNAL   PLoS One. 2012;7(3):e32372. doi: 10.1371/journal.pone.0032372. Epub 
            2012 Mar 5.
  PUBMED    22403649
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e32372. doi"; pages: " 
            10.1371/journal.pone.0032372. Epub 2012 Mar 5"
COMMENT     Sequence Label: pUAST attB BmOr2 (Y464A)
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    160..193
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    226..320
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        339..577
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     524..545
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 gaactgaaag tgacatccag tgtttgttcc ttgtgtagat gcatctcaaa aaaatggtgg
       61 gcataatagt gttgtttata tatatcaaaa ataacaacta taataataag aatacattta
      121 atttagaaaa tgcttggatt tcactggaac taggctagca taacttcgta taatgtatgc
      181 tatacgaagt tatgctagcg gatccaagct tgcatgcctg caggtcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga
      301 gcggagtact gtcctccgag cggagactct agcgagcgcc ggagtataaa tagaggcgct
      361 tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag
      421 ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta
      481 aagtgaatca attaaaagta accagcaacc aagtaaatca actgcaacta ctgaaatctg
      541 ccaagaagta attattgaat acaagaagag aactctgaat agggaattgg gaattcatga
      601 tgaccaaggt caagacgcag ggcctggtca cagacctgat gccctgcata agattactgc
      661 aagcagcggg ccacttctta ttcaattatc atgccgatac ctccggaatg aatatgctcc
      721 tacggaaaat atattcgagt gcccatgccg tgctcattgt cgtccactac atctgcatgg
      781 gcatcaacat ggcacaatac aaggacgaag tcaacgagct cacggcaaat accattacgg
      841 tcctgttttt cgctcacagc atcattaagt tggcattctt tgcgttcaac tctaaaagtt
      901 tttacagaac cctcgcagtg tggaatcagt ccaacagcca tccgttgttc acagaatcag
      961 atgcccgtta ccatcagatc tctttaagca aaatgcgaag gcttctttat ttcatctgtg
     1021 gcatgaccgt tttttcagta atcagctggg tcactt
//
