LOCUS       sequence-140345-         542 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  pSIREN-RetroQ vector containing shRNA sequence to BPTF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Mayes K, Elsayed Z, Alhazmi A, Waters M, Alkhatib SG, Roberts M, 
            Song C, Peterson K, Chan V, Ailaney N, Malapati P, Blevins T, Lisnic
            B, Dumur CI, Landry JW
  TITLE     BPTF inhibits NK cell activity and the abundance of natural 
            cytotoxicity receptor co-ligands.
  JOURNAL   Oncotarget. 2017 May 12;8(38):64344-64357. doi: 
            10.18632/oncotarget.17834. eCollection 2017 Sep 8.
  PUBMED    28969075
REFERENCE   2  (bases 1 to 542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.17834"; journalName: "Oncotarget"; date: 
            "2017-05-12- 12"; volume: "8"; issue: "38"; pages: "64344-64357"
COMMENT     Sequence Label: pSIREN-RetroQ-BPTF-sh3
FEATURES             Location/Qualifiers
     source          1..542
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(63..311)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(121..140)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(291..311)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(351..367)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(383..405)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tcacgcgtaa aaaacgacga tgactccgat tattctcttg aaataatcgg agtcatcgtc
       61 gcggatcctc gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt
      121 acggtaagca tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga
      181 aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta
      241 attctaatta tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga
      301 aataggccct cttcctgccc agatcttcaa ttccggcgcc tagagaagga gtgagggctg
      361 gataaaggga ggatcgaggc ggggtcgaac gaggaggttc aagggggaga gacggggcgg
      421 atggaggaag aggaggcgga ggcttagggt gtacaaaggg cttgacccag ggaggggggt
      481 caaaagccaa ggcttcccag gtcacgatgt aggggacctg gtctgggtgt ccatgcgggc
      541 ca
//
