LOCUS       sequence-140575-         844 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-J23114
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..87
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     promoter        115..149
                     /label=BBa_J23114
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.1"
     terminator      183..240
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(264..281)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(515..534)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaat ctagagacgc ataggtctca ctattttatg
      121 gctagctcag tcctaggtac aatgctagcg tacagagacc catgccgtct cattagctgc
      181 agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga
      241 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      301 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      361 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      421 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc
      481 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      541 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      601 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      661 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      721 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      781 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      841 ttga
//
