LOCUS       sequence-140839-       12460 bp    DNA     circular SYN 15-APR-2025
DEFINITION  lentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and
            Psd95 shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12460)
  AUTHORS   Schluter OM, Xu W, Malenka RC
  TITLE     Alternative N-terminal domains of PSD-95 and SAP97 govern 
            activity-dependent regulation of synaptic AMPA receptor function.
  JOURNAL   Neuron. 2006 Jul 6;51(1):99-111.
  PUBMED    16815335
REFERENCE   2  (bases 1 to 12460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2006-07-6"; volume: "51(1)"; pages: "99-111"
COMMENT     Sequence Label: FH95pUp95GW (B4)
FEATURES             Location/Qualifiers
     source          1..12460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2610..2824
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     2745..2764
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        2928..4138
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3975..3994
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      6390..6399
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6396..6398,6399..6401,6402..7115)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6396..6398,6399..6401,6402..7112)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6441..6462)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6702..6721)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7049..7070
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7158..7746
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7211..7231)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7629..7640)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8265..8445
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8471..8488)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8477..8701
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8747..9175
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8834..8853)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9044..9065
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9184..9204)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9189..9518
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9369..9504
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9431..9450
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9566..9613
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9632..10006
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10136..10257
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10173..10192)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10227..10246
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10306..10322)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10306..10322)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10319..10341)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10330..10346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10354..10360,10361..10378,10379..10384))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10399..10420
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10537..10554)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10708..11296)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10788..10807)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11467..12258,12259..12327))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12090..12109
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12328..12432)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcg tacgaattcg aacgctgacg tcatcaaccc gctccaagga
     2641 atcgcgggcc cagtgtcact aggcgggaac acccagcgcg cgtgcgccct ggcaggaaga
     2701 tggctgtgag ggacagggga gtggcgccct gcaatatttg catgtcgcta tgtgttctgg
     2761 gaaatcacca taaacgtgaa atgtctttgg atttgggaat cttataagtt ctgtatgaga
     2821 ccacagatcc ccggacatcc aggcacacaa gttcaagaga cttgtgtgcc tggatgtcct
     2881 ttttggaaag cttatcgaat taattaaccc gtgtcggctc cagatctggc ctccgcgccg
     2941 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     3001 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     3061 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     3121 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     3181 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     3241 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     3301 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     3361 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     3421 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcac aaaatggcgg
     3481 ctgttcccga gtcttgaatg gaagacgctt gtaaggcggg ctgtgaggtc gttgaaacaa
     3541 ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa
     3601 agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt
     3661 cactgactgg agaactcggg tttgtcgtct ggttgcgggg gcggcagtta tgcggtgccg
     3721 ttgggcagtg cacccgtacc tttgggagcg cgcgcctcgt cgtgtcgtga cgtcacccgt
     3781 tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct
     3841 ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga
     3901 cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta
     3961 tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt
     4021 ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca
     4081 gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacga
     4141 agcttgggct gcaggtcgac tctagaggat caattcgccc tttctagacc accatggact
     4201 gtctctgtat agtgacaacc aagaaatacc gctaccaaga tgaagacacg ccccctctgg
     4261 aacacagccc ggcccacctc cccaaccagg ccaattctcc ccctgtgatt gtcaacacgg
     4321 acaccctaga agccccagga tatgagttgc aggtgaatgg aacagagggg gagatggagt
     4381 atgaggagat cacattggaa aggggtaact caggtctggg cttcagcatc gcaggtggca
     4441 ctgacaaccc gcacatcggt gacgacccgt ccatttttat caccaagatc attcctggtg
     4501 gggctgcagc ccaggatggc cgcctcaggg tcaatgacag catcctgttt gtaaatgaag
     4561 tggatgttcg ggaggtgacc cattcagctg cggtggaggc cctcaaagag gcaggttcca
     4621 tcgttcgcct ctatgtcatg cgccggaaac ccccagccga aaaggtcatg gagatcaaac
     4681 tcatcaaagg gcctaaagga cttggcttca gcattgcggg gggcgttggg aaccagcaca
     4741 tccctggaga taacagcatc tatgtaacga agatcatcga aggaggtgct gcccacaagg
     4801 atggcaggtt gcagattgga gacaagatcc tggcggtcaa cagtgtgggg ctggaggacg
     4861 tcatgcacga ggatgccgtg gcagccctga agaacacata tgacgttgtg tacctaaagg
     4921 tggccaagcc cagcaatgcc tacctgagtg acagctatgc tcccccagac atcacaacct
     4981 cgtattctca gcacctggac aatgagatca gtcatagcag ctacttgggc actgactacc
     5041 ccacagccat gacccccact tcccctcggc gctactcccc tgtggccaag gacctgctgg
     5101 gggaggaaga cattccccgg gaaccaaggc ggatcgtgat ccatcggggc tccaccggcc
     5161 tgggcttcaa catcgtgggc ggcgaggatg gtgaaggcat cttcatctcc ttcatccttg
     5221 ctgggggtcc agccgacctc agtggggagc tacggaaggg ggaccagatc ctgtcggtca
     5281 atggtgttga cctccgcaat gccagtcacg aacaggctgc cattgccctg aagaatgcgg
     5341 gtcagacggt cacgatcatc gctcagtata aaccagaaga gtatagtcga ttcgaggcca
     5401 agatccatga tcttcgggaa cagctcatga atagtagcct aggctcaggg actgcatcct
     5461 tgcgaagcaa ccccaagagg ggcttctaca ttagggccct gtttgattac gacaagacca
     5521 aggactgcgg tttcttgagc caggccctga gcttccgctt cggggatgtg cttcatgtca
     5581 ttgacgctgg tgacgaagag tggtggcaag cacggcgggt ccactccgac agtgagaccg
     5641 acgacattgg cttcattccc agcaaacggc gggtcgagcg acgagagtgg tcaaggttaa
     5701 aggccaagga ctggggctcc agctctggat cacagggtcg agaagactcg gttctgagct
     5761 atgagacggt gacccagatg gaagtgcact atgctcgtcc catcatcatc cttggaccca
     5821 ccaaagaccg tgccaacgat gatcttctct ccgagttccc cgacaagttt ggatcctgtg
     5881 tccctcatac gacacgtcct aagcgggaat atgagataga cggccgggat taccactttg
     5941 tctcctcccg ggagaaaatg gagaaggata ttcaagcgca taagttcatt gaggctggcc
     6001 agtacaacag ccacctctat gggaccagcg tccagtctgt gcgagaggta gcagagcagg
     6061 ggaagcactg catcctcgat gtctcggcca atgccgtgcg gcggctgcag gcggcccacc
     6121 tgcaccccat cgccatcttc atccgtcccc gctccctgga gaatgtgcta gagatcaata
     6181 agcggatcac agaggagcaa gcccggaaag ccttcgacag agccacgaag ctggagcagg
     6241 agttcacaga gtgcttctca gccatcgtag agggcgacag ctttgaagag atctatcaca
     6301 aagtgaaacg tgtcattgaa gacctctcag gcccctacat ctgggtccca gcccgagaga
     6361 gactcggtac cgcgggcccg ggatccatcg ccaccatggt gagcaagggc gaggagctgt
     6421 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     6481 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     6541 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     6601 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     6661 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     6721 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     6781 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     6841 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     6901 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     6961 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     7021 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     7081 ggatcactct cggcatggac gagctgtaca agtaagctag ctcgagctag aattaattcg
     7141 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7201 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7261 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7321 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7381 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     7441 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     7501 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     7561 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     7621 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     7681 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     7741 ccccgcatcg ataccgtcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     7801 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     7861 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7921 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     7981 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     8041 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     8101 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     8161 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     8221 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     8281 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8341 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8401 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc
     8461 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     8521 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     8581 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     8641 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     8701 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     8761 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     8821 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     8881 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     8941 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     9001 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     9061 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     9121 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     9181 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     9241 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     9301 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     9361 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     9421 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     9481 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     9541 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     9601 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     9661 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     9721 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     9781 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     9841 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     9901 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     9961 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
    10021 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
    10081 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
    10141 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    10201 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    10261 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
    10321 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    10381 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    10441 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    10501 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    10561 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    10621 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    10681 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    10741 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10801 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10861 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    10921 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    10981 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    11041 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    11101 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    11161 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    11221 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    11281 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    11341 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    11401 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    11461 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    11521 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    11581 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    11641 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    11701 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    11761 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    11821 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    11881 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    11941 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    12001 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    12061 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    12121 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    12181 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    12241 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    12301 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    12361 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    12421 aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
