LOCUS       sequence-141212-         832 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  pAAV vector to express oG (optimized Glycoprotein) in a 
            Cre-dependent manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Kim EJ, Jacobs MW, Ito-Cole T, Callaway EM
  TITLE     Improved Monosynaptic Neural Circuit Tracing Using Engineered Rabies
            Virus Glycoproteins.
  JOURNAL   Cell Rep. 2016 Apr 26;15(4):692-699. doi: 
            10.1016/j.celrep.2016.03.067. Epub 2016 Apr 14.
  PUBMED    27149846
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.067"; journalName: "Cell Rep"; date: 
            "2016-04-26- 26"; volume: "15"; issue: "4"; pages: "692-699"
COMMENT     Sequence Label: pAAV-CAG-FLEX-oG-WPRE-SV40pA
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..36
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    99..132
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ccataacttc gtataaagta tcctatacga agttatatca aaataggaag accaatgctt
       61 caccatcgac ccgaattgcc aagcatcacc atcgacccat aacttcgtat aatgtatgct
      121 atacgaagtt atactaggga tccccttact tgtacattag agccgtgtct cgcccccaga
      181 tttatgactt tcccaactgc tgatgatttt accgctctgg ggtgtgacag acacttctct
      241 accggtcccc ctcagattgt gctgagttgg ctcggagcgg ttcactcgtc gccagcaggt
      301 catcaggaaa atgatcagca tgagagcagt cagtgctcca gcggagagca gcacgtactt
      361 gccccaatta ggcagtccga gatcgacgcc actgatccgt tcgtgcacgt cgggcaggtg
      421 gacctccaca aaatcttcgg cctcgtcgcc gtttttgaac actgtgcttg ggtctgcgag
      481 aggatgcatc aggggaatga cgcttgacac gagcagttcc atgtgctgct ggagcagact
      541 agactgcatc tcggggatca gcacgttacc atctggcccc agaatgatac cattaaagaa
      601 gaccccgttc acatgtgggt gacatcgacc cccgactcgc aggcatccct tactaggaat
      661 gatttcattc caggttctca cagatttata atgggcgtct gcctccatca gtgtcttgtt
      721 aaagatggtg tatgcttttc cgaagccagg gacgagcttc ctcaggtggc tcagcctcct
      781 gaagctcacg ctcttggtag tcataattga ctccagagca tcgagacatt cc
//
