LOCUS       sequence-141441-         981 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  RORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of 
            mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Wang S, Sengel C, Emerson MM, Cepko CL
  TITLE     A gene regulatory network controls the binary fate decision of rod 
            and bipolar cells in the vertebrate retina.
  JOURNAL   Dev Cell. 2014 Sep 8;30(5):513-27. doi: 
            10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
  PUBMED    25155555
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.07.018"; journalName: "Dev Cell"; date: 
            "2014-09-8- 8"; volume: "30"; issue: "5"; pages: "513-27"
COMMENT     Sequence Label: CAG-mCherry-RORbshRNA2
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..719
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(154..172)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     422..441
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     ncRNA           725..751
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(938..957)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 aagaattgat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
       61 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
      121 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
      181 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
      241 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
      301 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
      361 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
      421 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
      481 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
      541 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
      601 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
      661 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag
      721 cggcctggag gcttgctgaa ggctgtatgc tgttctgaca cagctccatg aaggttttgg
      781 ccactgactg accttcatgg ctgtgtcaga acaggacaca aggcctgtta ctagcactca
      841 catggaacaa atgcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg
      901 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
      961 atggggacat catgaagccc c
//
