LOCUS       sequence-141583-         640 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expression of GFP-PP-RPN2 (925-953) fusion in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   VanderLinden RT, Hemmis CW, Yao T, Robinson H, Hill CP
  TITLE     Structure and energetics of pairwise interactions between proteasome
            subunits RPN2, RPN13, and ubiquitin clarify a substrate recruitment 
            mechanism.
  JOURNAL   J Biol Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 
            10.1074/jbc.M117.785287.
  PUBMED    28442575
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2017 Apr 25. pii: jbc.M117.785287. doi: 
            10.1074/jbc.M117.785287."
COMMENT     Sequence Label: pET151-GFP-PP-RPN2 (925-953)
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(130..153)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(196..217)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     545..564
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctgag ctcgcccttt taatcatcaa tatactcaaa
       61 tggttctggg ggttctggct cttgttcctc ctcctcgatt tttgggccat gtgctgccac
      121 aggttccacc ggcccctgga acagaacttc cagcttatac agctcgtcca tgccgagagt
      181 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
      241 tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc
      301 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
      361 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
      421 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
      481 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      541 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
      601 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
//
