LOCUS       sequence-141650-       11065 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Translation reporter with Xbp1 pause site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11065)
  AUTHORS   Yan X, Hoek TA, Vale RD, Tanenbaum ME
  TITLE     Dynamics of Translation of Single mRNA Molecules In Vivo.
  JOURNAL   Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
  PUBMED    27153498
REFERENCE   2  (bases 1 to 11065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-5- 5";
            volume: "165"; issue: "4"; pages: "976-89"
COMMENT     Sequence Label: pcDNA4TO-24xGCN4_v4-kif18b-Xbp1-24xPP7
FEATURES             Location/Qualifiers
     source          1..11065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      984..993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1059..1115
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1131..1187
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1203..1259
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1275..1331
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1347..1403
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1419..1475
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1491..1547
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1563..1619
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1635..1691
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1707..1763
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1779..1835
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1851..1907
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1923..1979
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1995..2051
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2067..2123
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2139..2195
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2211..2267
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2283..2339
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2355..2411
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2427..2483
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2499..2555
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2571..2627
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2715..2771
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(7076..7093)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7082..7306
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7352..7780
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7439..7458)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7649..7670
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7789..7809)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7794..8123
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7974..8109
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8036..8055
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8171..8218
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8237..8611
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8741..8862
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8778..8797)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8832..8851
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8911..8927)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8911..8927)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8924..8946)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8935..8951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8959..8965,8966..8983,8984..8989))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9004..9025
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9142..9159)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9313..9898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9393..9412)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10069..10860,10861..10929))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10692..10711
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10930..11034)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgccacca tgggcgccat catacacgcg gccgcaatca
     1021 tcggtccggg tggatctgga ggtggaggtt ctggaggaga agaacttttg agcaagaatt
     1081 atcatcttga gaacgaagtg gctcgtctta agaaaggttc tggcagtgga gaagaactgc
     1141 tttcaaagaa ttaccacctg gaaaatgagg tagctagact gaaaaagggg agcggaagtg
     1201 gggaggagtt gctgagcaaa aattatcatt tggagaacga agtagcacga ctaaagaaag
     1261 ggtccggatc gggtgaggag ttactctcga aaaattatca tctcgaaaac gaagtggctc
     1321 ggctaaaaaa gggcagtggt tctggagaag agctattatc taaaaactac cacctcgaaa
     1381 atgaggtggc acgcttaaaa aagggaagtg gcagtggtga agagctacta tccaagaatt
     1441 atcatcttga gaacgaggta gcgcgtttga agaagggttc cggctcagga gaggaactgc
     1501 tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt aaaaaaggga tcgggcagtg
     1561 gtgaggaact actttcaaag aattaccacc tcgaaaacga agtagctcga ttaaagaaag
     1621 gttcagggtc gggtgaagaa ttactgagta aaaattatca tctggaaaat gaggtagcga
     1681 gactaaaaaa ggggagtggt tctggcgaag agttgctatc gaaaaattat catcttgaga
     1741 acgaagttgc taggctcaaa aagggctcag gctcaggcga ggagttgctc tcgaaaaact
     1801 accacttgga aaatgaggtc gcgaggttga aaaaggggag cgggtcgggc gaggagttat
     1861 tgagcaaaaa ctatcattta gagaacgaag tcgcgcgctt aaagaaaggc tcgggctcgg
     1921 gcgaagaact cttatcgaag aactaccacc tcgaaaatga ggtcgccagg ttgaaaaagg
     1981 gcagtggcag cggggaggaa ctcttgagca agaactacca cttggagaat gaggtcgcga
     2041 gattgaagaa agggtcgggg agcggcgagg aattgctcag caagaattat catttggaga
     2101 acgaagtcgc caggctcaag aaaggctcgg ggtcggggga ggaattgttg agtaaaaact
     2161 accacttgga aaatgaagtc gccaggctca aaaaagggag tgggagcggc gaagagttat
     2221 tgagcaaaaa ttaccacttg gagaacgaag tggcaaggct caagaaaggg agcggcagcg
     2281 gggaggagct cttatcgaag aactaccact tagagaatga agtcgcccgc ttgaagaaag
     2341 gctcggggag cggggaagag ctcttgagca agaactacca cttggaaaat gaggtggcgc
     2401 gcttgaagaa agggagcggg agcggggaag agttactatc taagaattat catctcgaga
     2461 acgaggtggc tcgactaaag aagggctccg gcagtgggga ggaactcctg tcgaagaact
     2521 atcatcttga aaatgaggtt gcaagactta aaaaggggtc cggatcaggt gaggaactac
     2581 tcagtaagaa ttaccacctg gaaaacgaag ttgcacgttt gaagaaagga tcaggatcag
     2641 gcgaagaact gctctcaaaa gattatcatt tggaaaatga ggttgcacgt ttaaaaaagg
     2701 gaagtggcag tggtgaggaa cttctgtcga aaaattatca tctcgagaat gaagtagccc
     2761 gacttaaaaa gggttctggc tcgggtcagc ggccgcaccg gtcagcagtg gaggacagca
     2821 cgctgcaagt agtggtacgg gtgcggcccc ccacccctcg ggagctggac agtcagcggc
     2881 ggccagtggt tcaggtggtg gacgagcggg tgctggtgtt taaccctgag gagcccgatg
     2941 gagggttccc tggcctgaaa tggggtggca cccatgatgg ccccaagaag aagggcaaag
     3001 acctgacgtt tgtctttgac cgggtctttg gcgaggcggc cacccaacag gacgtgttcc
     3061 agcacaccac gcacagcgtc ctggacagct tcctccaggg ctacaactgc tcagtgtttg
     3121 cctacggggc caccggggct gggaagacac acaccatgct gggaagggag ggggaccccg
     3181 gcatcatgta cctgaccacc gtggaactgt acaggcgcct ggaggcccgc cagcaggaga
     3241 agcacttcga ggtgctcatc agctaccagg aggtgtataa tgaacagatc catgacctcc
     3301 tggagcccaa ggggcccctt gccatccgcg aggaccccga caagggggtg gtggtgcaag
     3361 gactttcttt ccaccagcca gcctcagccg agcagctgct ggagatactg accaggggga
     3421 accgtaaccg cacgcagcac cccactgatg ccaacgcgac ttcctcccgc tcccatgcca
     3481 tcttccagat ctttgtgaag cagcaggacc gggttccagg actgacccag gctgtccagg
     3541 tggccaagat gagcctgatt gacctggctg gctcagagcg ggcatccagc acccatgcga
     3601 agggggagcg gctgcgggag ggggccaaca tcaaccgctc tctgctggcg ctcatcaacg
     3661 tcctcaatgc cttggccgat gcaaagggcc gcaagaccgc tgtgccctac gcggacagcg
     3721 cactgacccg cctgctcaaa gactccctcg ggggcaactg ccgcacagtg atgatcgctg
     3781 ccatcagccc ctccagcctg acctacgagg acacgtataa taccctcaaa tatgccgacc
     3841 gggccaagga gatcaggctc tcgctgaaga gcaatgtgac cagcctggac tgtcacatca
     3901 gccagtatgc taccatctgc caacagctcc aggctgaggt agccgctctg aggaagaagc
     3961 tccaagtgta tgagggggga ggccagcccc caccacagga cctcccagga tctcccaagt
     4021 cgggaccacc accagaacac cttcccagct cccccttgcc accccaccct cccagccagc
     4081 cctgcacccc agagctccct gcagggccta gagcccttca agaggagagt ctggggatgg
     4141 aggcccaggt ggagagggcc atggaaggga actcttcaga ccaggagcag tccccagagg
     4201 atgaggatga aggcccagct gaggaggttc caacccagat gccagagcag aaccccacac
     4261 atgcactgcc agagtcccct cgcctgaccc tgcagcccaa gccagtcgtg ggccacttct
     4321 cagcacggga actggatggg gaccgttcta agcggttggc cctaaaggtg ctgtgcgttg
     4381 cccagcggca gtactccctg ctccaagcag ccaacctcct gacgcccgac atgatcacag
     4441 agtttgagac cctacagcag ctggtgcaag aggaaaaaat tgagcctggg gcagaggcct
     4501 tgaggacttc aggcctggcc aggggggcac ctctggctca ggagctgtgt tcagagtcaa
     4561 agcctccagg atacactggc cctgtgaccc ggactatggc gaggcgactg agtggccccc
     4621 tgcacaccct gggaatcccg cctggaccca actgcacccc agcccagggg tcccgatggc
     4681 ccatggagaa gaagaggagg agaccaagcg ccttggaggc agacagtccc atggccccaa
     4741 agcggggcac caagcgccag cgccagtcct tcctgccctg cctaaggaga gggtctctgc
     4801 ctgacaccca accttcacag gggcccagca cccccaaagg agaaagggcc tcctccccct
     4861 gccattcccc tcgcgtttgc ccagccacag tcatcaaaag ccgggtgccc ctgggccctt
     4921 ccgccatgca gaactgctcc accccgctgg ctctgcccac tcgagacctc aatgccacct
     4981 ttgatctctc tgaggagcct ccctcaaagc ccagtttcca tgaatgcatt ggctgggaca
     5041 aaatacccca ggagctgagc aggctggacc agcccttcat ccccagggca cctgtgcccc
     5101 tgttcaccat gaagggcccc aagccaacat cttccctccc tgggacctct gcctgcaaga
     5161 agaagcgcgt tgcgagttcc tcagtctccc atggccgcag ccgcatcgcc cgcctcccca
     5221 gcagcacttt gaagaggcca gctgggcccc ttgtactccc agagctgccc ttgagtcccc
     5281 tgtgccctag caaccggagg aatggaaagg acctcatcag ggtggggaga gcactctcag
     5341 cagggaacgg cgtcaccaag gtgtccgata aggaccccgt gccctaccaa cctccattcc
     5401 tgtgtcaatg gggccgacat cagccagcct ggaaaccctt gatgaatggc ggcggcgccg
     5461 ccagcgccaa agccatctaa cgacacgatt cgaagatcgc atcgctagcc cggttgtaca
     5521 ggtaccggat cctaaggtac ctaattgcct agaaaggagc agacgatatg gcgtcgctcc
     5581 ctgcaggtcg actctagaaa ccagcagagc atatgggctc gctggctgca gtattcccgg
     5641 gttcattaga tcctaaggta cctaattgcc tagaaaggag cagacgatat ggcgtcgctc
     5701 cctgcaggtc gactctagaa accagcagag catatgggct cgctggctgc agtattcccg
     5761 ggttcattag atcctaaggt acctaattgc ctagaaagga gcagacgata tggcgtcgct
     5821 ccctgcaggt cgactctaga aaccagcaga gcatatgggc tcgctggctg cagtattccc
     5881 gggttcatta gatcctaagg tacctaattg cctagaaagg agcagacgat atggcgtcgc
     5941 tccctgcagg tcgactctag aaaccagcag agcatatggg ctcgctggct gcagtattcc
     6001 cgggttcatt agatcctaag gtacctaatt gcctagaaag gagcagacga tatggcgtcg
     6061 ctccctgcag gtcgactcta gaaaccagca gagcatatgg gctcgctggc tgcagtattc
     6121 ccgggttcat tagatcctaa ggtacctaat tgcctagaaa ggagcagacg atatggcgtc
     6181 gctccctgca ggtcgactct agaaaccagc agagcatatg ggctcgctgg ctgcagtatt
     6241 cccgggttca ttagatccta aggtacctaa ttgcctagaa aggagcagac gatatggcgt
     6301 cgctccctgc aggtcgactc tagaaaccag cagagcatat gggctcgctg gctgcagtat
     6361 tcccgggttc attagatcct aaggtaccta attgcctaga aaggagcaga cgatatggcg
     6421 tcgctccctg caggtcgact ctagaaacca gcagagcata tgggctcgct ggctgcagta
     6481 ttcccgggtt cattagatcc taaggtacct aattgcctag aaaggagcag acgatatggc
     6541 gtcgctccct gcaggtcgac tctagaaacc agcagagcat atgggctcgc tggctgcagt
     6601 attcccgggt tcattagatc ctaaggtacc taattgccta gaaaggagca gacgatatgg
     6661 cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg ctggctgcag
     6721 tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc agacgatatg
     6781 gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc gctggctgca
     6841 gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaggag cagacgatat
     6901 ggcgtcgctc cctgcaggtc gactctagaa accagcagag catatgggct cgctggctgc
     6961 agtattcccg ggttcattag atctcgcgaa gggcgaattc acgaggcgcg cccggatcgc
     7021 gatcgcaatc ttaattaact cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     7081 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     7141 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     7201 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     7261 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     7321 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     7381 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7441 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7501 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7561 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7621 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7681 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7741 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     7801 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7861 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7921 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7981 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8041 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     8101 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     8161 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     8221 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     8281 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     8341 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     8401 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     8461 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     8521 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     8581 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     8641 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     8701 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     8761 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8821 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     8881 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8941 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9001 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9061 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9121 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9181 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9241 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9301 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9361 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9421 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9481 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9541 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9601 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9661 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9721 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     9781 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9841 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9901 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9961 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10021 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10081 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10141 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10201 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10261 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10321 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10381 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10441 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10501 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10561 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10621 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10681 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10741 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10801 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10861 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10921 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10981 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11041 tccccgaaaa gtgccacctg acgtc
//
