LOCUS       sequence-141916-        5972 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Can be used to drive pan neuronal expression in zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: p5E-elavl3
FEATURES             Location/Qualifiers
     source          1..5972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        690..708
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3946..3962)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3946..3962)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(3999..4017)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    4018..4141
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(4214..4232)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4237..4253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4237..4253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4355..4374)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4366..5175
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4433..4452)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5322..5910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5811..5830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggt aatacgactc actatagggc gaattgggta
      721 ccgggccccc cctcgagctc aagcttcgaa ttcactaatt tgaatttaaa tgcattatct
      781 ttctattcct aaagaccttg ggtgaccaaa atcttatttt aataaataaa actgtttatt
      841 aaaacttttt tgtttcaaag aaccatatgt atagtgaaat ttataaaaat atcaattttt
      901 aaaaagctgg tgtactcatt tatgttatga actctaaaac catatactga ctgcaagtga
      961 tgatgtatag agtgatgttt acgagtaaac atatttagtt gtatacatcc tactgagcac
     1021 attttgatgt atgaaataac attacaagct ttatccaaat taagccattt taaaacactg
     1081 ccaattgaaa atacaaatcc tggaaaaaat cgtctttagc gcagtcattt gagccatcct
     1141 aatccgttac ctcagaccat aataagaagg gataacacta gctgtagcaa tggaacacat
     1201 ctgtttcaca caatcatatc tcctgcgccg gtgctaagca gattcagcgt gatcataaca
     1261 tgctttccac tcataaatgt aaatttacaa tttgcacatg taaaacagac acttttgaga
     1321 tattggataa aaaaacaaga gtatattgct tagtttcatc caccagtcat ccccacagcg
     1381 tttggaaggc cataaaaagt gtctaaaatc aatgatcatt gaaagagcac aagagagact
     1441 cttacgctgt aatgccactg gggacaaaag tgacagtctc ttaatgggct cttctggagg
     1501 ggctcctgaa cattaaaaat tatcagcgaa attaccgaaa gagcttcaag caactggcat
     1561 gcttgatcct ctgcgtcggg gcggtgaata ggtgcttcag atgccctctt acccacgggc
     1621 tggattcagc tgccccgcta ccagcggaga ccccctaatg agcctctgca attaagttta
     1681 ttcatgttaa gtgtgaacgg ggtgcgtgcg gaactgtggg cagctaacag acctgggttc
     1741 tttgtgccac aagtgctgcc tttattcggc tcacaaagca gaaaacaaca cccgcaccta
     1801 taatggcgcc ctcggctggg tctaagaaac gtggcgagtt gacagagcag agtgggcggg
     1861 gttaagacag actgacagcg ggacccatct ccatcctctt attaacgctt aacgagtgcc
     1921 ttcctcatgc aatattcatc gccactaata tcatccaagc tctgagctga gctggccact
     1981 tatgtaaggc aattatgtaa aatatcagac agggcccaca ctcagaatct gactggggta
     2041 gagacgcggg acgagaaccg agagcaagaa ctgaaagtga aagtgaccac taaagggagg
     2101 agaggacaga ggggcaggat gtgtcaagat taccagagaa cacttggcca gaaatgcgca
     2161 accattggag ctctccggat tacccaaagg ttaacgagtt tgaacgcctc tgcccactcg
     2221 cccatctctg atggtttccc aagaactcct caagcaaaat atatataatt gtgtgtatta
     2281 tgcacagaca cgagaaaatg ctgtttttct gatctgcatt acagcacatt tgcccgccaa
     2341 cgacaatacc acccactcgg tacctcgctg actcctgatg cctgatacct gcgcggtgac
     2401 tgtctacaat ctgcataatc aagagaagtt gtgttgaaga cgagcgccac acaaccgttt
     2461 ccacaaggtc acccaaggcc ggtgcagatg taggtgaggt ctccataaac agactgaaat
     2521 aaacacatcc tccgctggga acaacaaccc cctcacgcct catgcatttc cataagccta
     2581 catgcatctc ttccaactta tggagactcg cacctaccaa catccgcaca acaaagatat
     2641 acagagcgcg ctccctcagg tcaaggccct gtgggggtct gtgcagaaat aggtcatttg
     2701 tcacacatca agtcctgggg caggagatgc attatagatg agaccaaaca gcctgtctcg
     2761 gtgagctcta cccactccct gagactagaa atgggggaag ggagcttgag ataacaaccg
     2821 ctgcaatcac tgtgtcgatg tttaatatca gcaccaaccg ggaacaataa ggagatgggt
     2881 gcattcatgt tcacatctta ccagtcaagt atcatcgaac cggcttgata accacacctc
     2941 gtgtaatagc tgagcagata gttgtcattt taaagcgttg gcctttgtcg attatgtaat
     3001 gcgcacattc aacacatggt aatatagaaa cggttatgtc gaggttgttt tgtccagaga
     3061 tgaccttcac acagttacag ccgctctgca tccacacaaa tggaggactt aatcgtggac
     3121 tgcattctta gaaatgatct acaaagacaa ataatgtgaa atcaagaaag gacaaaattt
     3181 aagtaagggg atgagggaga gagagaacga ggggcaagga gaaagcatgg ctcctgtctt
     3241 tttctgcacc catctgttcg gagtgcaggt ggagctctat tcactcagct ctgcatgtgt
     3301 gtttgggggg ggcaggaaga aagggagggc aaaaggaaga gtggagagat ggtgggggct
     3361 ggagggatgg ggggttctcg gtgatctctc ctgaagggga taatgggaga gcagcgcttt
     3421 gcaatggctg ccatgtagta ccctccctgc acaattagcc aatcagcagc aagctctgcc
     3481 agccagaagg acacataaaa gaagaacatt gcagcagagg cacagaagga gcctgcgagg
     3541 agctgggaaa tacacacaca acagcagaac cacaacaccc tcccctggac acaccctact
     3601 ggggatcact gcttttcttt ttttctgaac catcgcccac gccacacgga gagaaatctc
     3661 tctctcatca tcatcctgaa gaaaaccccc ttatcctcat tttcacactg ctgaggaaaa
     3721 cctacaatcg cacgggctga gatttcctgg cgaagactgt cctttttcct ttttcttttt
     3781 ttttcttttc ctttggaaac tgacatttgc atttctccat tccaagccac ggcgtaataa
     3841 tatctgcaag ccagcctgaa gacctgcaaa tcgaaggacc tagatcacta tcatctttgt
     3901 acgtcaagaa atcgaattct gcagtcgacg gtaccgcggg cccgggatcc actagttcta
     3961 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattcaa
     4021 gtttgtacaa aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     4081 agattttgca taaaaaacag actacataat actgtaaaac acaacatatg cagtcactat
     4141 gaaccaacta cttagatggt attagtgacc tgtagaattc gagctctaga gctgcagggc
     4201 ggccgcgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     4261 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     4321 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     4381 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     4441 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     4501 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     4561 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     4621 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     4681 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     4741 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     4801 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     4861 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     4921 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     4981 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     5041 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     5101 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     5161 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     5221 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     5281 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5341 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5401 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5461 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5521 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5581 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5641 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5701 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5761 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5821 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5881 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5941 ctggcctttt gctggccttt tgctcacatg tt
//
