LOCUS       sequence-142101-         980 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  eF1alpha::puroR::mCherry::T2A::eGFP::d1ODC::wPRE has d1ODC degron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Arribere JA, Cenik ES, Jain N, Hess GT, Lee CH, Bassik MC, Fire AZ
  TITLE     Translation readthrough mitigation.
  JOURNAL   Nature. 2016 Jun 1;534(7609):719-723. doi: 10.1038/nature18308.
  PUBMED    27281202
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-06-1- 1"; volume: "534"; issue: "7609"; pages:
            "719-723"
COMMENT     Sequence Label: pJA317
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    94..127
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(336..357)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     685..704
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     944..965
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat
       61 tgtacctagt ggaaccggaa cccttaaaca tgtataactt cgtataatgt atgctatacg
      121 aagttattag gtccctcgac gaattcttat tacctgcagg cctacacatt gatcctagca
      181 gaagcacagg ctgcagggtg acggtccatc ccgctctcct gggcacaaga catgggcagc
      241 gtgccatcat cctgcgccgc caccgccggc gggaagccat ggctgcggcc gcacttgtac
      301 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
      361 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
      421 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
      481 acgctgccgt cctcgatgtt gtggcggatc ttgaagttcg ccttgatgcc gttcttctgc
      541 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
      601 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
      661 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
      721 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
      781 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
      841 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
      901 tcgccctcgc cctcgccaga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
      961 accaggatgg gcaccacccc
//
