LOCUS       sequence-142211-         928 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Vector for SICLOPPS-mediated protein circularization using the 
            gp41-1 intein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Waldhauer MC, Schmitz SN, Ahlmann-Eltze C, Gleixner JG, Schmelas CC,
            Huhn AG, Bunne C, Buscher M, Horn M, Klughammer N, Kreft J, Schafer 
            E, Bayer PA, Kramer SG, Neugebauer J, Wehler P, Mayer MP, Eils R, Di
            Ventura B
  TITLE     Backbone circularization of Bacillus subtilis family 11 xylanase 
            increases its thermostability and its resistance against 
            aggregation.
  JOURNAL   Mol Biosyst. 2015 Nov 10;11(12):3231-43. doi: 10.1039/c5mb00341e.
  PUBMED    26434634
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2015-11-10- 10"; volume: "11"; issue: "12"; 
            pages: "3231-43"
COMMENT     Sequence Label: pCIRCgp41-1
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(257..314)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    315..335
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(643..670)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(686..757)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctgtgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcct
      241 gcataacgcg aagtaatctt ttcggtttta aagaaaaagg gcagggtggt gacaccttgc
      301 ccttttttgc cggactgcag cggccgctac tagtactatc agtcgtgcat ttcaccgatt
      361 tattattctt taacatacag acacatacct tctttcagac caccggagat gttcatttca
      421 ccagtctgcg tcggaaacag gtgttcttcg gaacagatga tttccttgcc atcttccagg
      481 gtgattttgt aagacttttt tttagatttc gggaagacgt tcagaacttc gttgtaaccc
      541 gtgttgctca gtaccagatc accgacctgg atgttggaaa tttccttcat accctgcggg
      601 gtctgaacct gggttttcag atccaagcat gagacctata aacgcagaaa ggcccacccg
      661 aaggtgagcc agtgtgactc tagtagagag cgttcaccga caaacaacag ataaaacgaa
      721 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggctcta gtagcgatct
      781 acactagcac tatcagcgtt attaagcacc ggtggagtga cgaccttcag cacgttcgta
      841 ctgttcaacg atggtgtagt cttcgttgtg ggaggtgatg tccagtttga tgtcggtttt
      901 gtaagcaccc ggcagctgaa ccggtttt
//
