LOCUS       sequence-142226-         891 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Protein Quantitation Reporter (PQR) to quantify a protein of 
            interest in insect cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Lo CA, Kays I, Emran F, Lin TJ, Cvetkovska V, Chen BE
  TITLE     Quantification of Protein Levels in Single Living Cells.
  JOURNAL   Cell Rep. 2015 Dec 22;13(11):2634-44. doi: 
            10.1016/j.celrep.2015.11.048. Epub 2015 Dec 10.
  PUBMED    26686644
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.11.048"; journalName: "Cell Rep"; date: 
            "2015-12-22- 22"; volume: "13"; issue: "11"; pages: "2634-44"
COMMENT     Sequence Label: pJFRC7-RFPnls-PQRv2-mCD8:GFP
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     149..170
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          217..283
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
ORIGIN
        1 caattcaatt caaacaagca aagtgaacac gtcgctaagc gaaagctaag caaataaaca
       61 agcgcagctg aacaagctaa acaatctgca gtaaagtgca agttaaagtg aatcaattaa
      121 aagtaaccag caaccaagta aatcaactgc aactactgaa atctgccaag aagtaattat
      181 tgaatacaag aagagaactc tgaatagatc taaaaggtag gttcaaccac tgatgcctag
      241 gcacaccgaa acgactaacc ctaattctta tcctttactt caggcggccg catggtgtct
      301 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
      361 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
      421 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
      481 accagcttca tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgatttc
      541 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
      601 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
      661 aagatcagag gggtgaactt cccatccaac tgccctgtga tgcagaagaa aacactcggc
      721 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaaccgac
      781 atggccctga agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga
      841 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga c
//
