LOCUS       sequence-142261-        9935 bp    DNA     circular SYN 14-APR-2025
DEFINITION  retroviral expression plasmid for human NFATc2/C with C-terminal 
            BirA-biotinylation signal and TEV celavage site .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 9935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMIG-hNFATc2/C - AVITEV
FEATURES             Location/Qualifiers
     source          1..9935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             43..560
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    624..965
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1032..1448
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1378..1400
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1416..1432
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      3651..3660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4266..4286
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4299..4343
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     4376..4392
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4377..4393
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4412..4978
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4428..4980
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4572..4589)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4799..4818
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4992..4994,4995..4997,4998..5711)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4992..4994,4995..4997,4998..5708)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5037..5058)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5298..5317)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5645..5666
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5802..6317
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6486..6502)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6486..6502)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6499..6521)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6510..6526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6534..6540,6541..6558,6559..6564))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6579..6600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6717..6734)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6888..7476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6968..6987)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7647..8438,8439..8507))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8270..8289
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8508..8612)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8680..8698
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8736..8758)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8858..8877
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8900..8920)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8905..9234
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9085..9220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9147..9166
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9282..9329
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     9523..9545
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9537..9554
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9538..9554
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9757..9776
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
       61 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg
      121 agaatagaga agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca
      181 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
      241 tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
      301 acctgaaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
      361 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
      421 agtcctccga tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt
      481 tgcatccgaa tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc
      541 cacctcgggg gtctttcatt tggaggttcc accgagattt ggagacccct gcctagggac
      601 caccgacccc cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct
      661 ttgtgcgtgt ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact
      721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
      901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
      961 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     1021 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1081 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1141 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1201 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1261 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1321 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1381 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1441 taggcgccgg aattagatct gcggccgcgg atttaaatcc caccatgaac gcccccgagc
     1501 ggcagcccca acccgacggc ggggacgccc caggccacga gcctgggggc agcccccaag
     1561 acgagcttga cttctccatc ctcttcgact atgagtattt gaatccgaac gaagaagagc
     1621 cgaatgcaca taaggtcgcc agcccaccct ccggacccgc ataccccgat gatgtcctgg
     1681 actatggcct caagccatac agcccccttg ctagtctctc tggcgagccc cccggccgat
     1741 tcggagagcc ggatagggta gggccgcaga agtttctgag cgcggccaag ccagcagggg
     1801 cctcgggcct gagccctcgg atcgagatca ctccgtccca cgaactgatc caggcagtgg
     1861 ggcccctccg catgagagac gcgggcctcc tggtggagca gccgcccctg gccggggtgg
     1921 ccgccagccc gaggttcacc ctgcccgtgc ccggcttcga gggctaccgc gagccgcttt
     1981 gcttgagccc cgctagcagc ggctcctctg ccagcttcat ttctgacacc ttctccccct
     2041 acacctcgcc ctgcgtctcg cccaataacg gcgggcccga cgacctgtgt ccgcagtttc
     2101 aaaacatccc tgctcattat tcccccagaa cctcgccaat aatgtcacct cgaaccagcc
     2161 tcgccgagga cagctgcctg ggccgccact cgcccgtgcc ccgtccggcc tcccgctcct
     2221 catcgcctgg tgccaagcgg aggcattcgt gcgccgaggc cttggttgcc ctgccgcccg
     2281 gagcctcacc ccagcgctcc cggagcccct cgccgcagcc ctcatctcac gtggcacccc
     2341 aggaccacgg ctccccggct gggtaccccc ctgtggctgg ctctgccgtg atcatggatg
     2401 ccctgaacag cctcgccacg gactcgcctt gtgggatccc ccccaagatg tggaagacca
     2461 gccctgaccc ctcgccggtg tctgccgccc catccaaggc cggcctgcct cgccacatct
     2521 acccggccgt ggagttcctg gggccctgcg agcagggcga gaggagaaac tcggctccag
     2581 aatccatcct gctggttccg cccacttggc ccaagccgct ggtgcctgcc attcccatct
     2641 gcagcatccc agtgactgca tccctccctc cacttgagtg gccgctgtcc agtcagtcag
     2701 gctcttacga gctgcggatc gaggtgcagc ccaagccaca tcaccgggcc cactatgaga
     2761 cagaaggcag ccgaggggct gtcaaagctc caactggagg ccaccctgtg gttcagctcc
     2821 atggctacat ggaaaacaag cctctgggac ttcagatctt cattgggaca gctgatgagc
     2881 ggatccttaa gccgcacgcc ttctaccagg tgcaccgaat cacggggaaa actgtcacca
     2941 ccaccagcta tgagaagata gtgggcaaca ccaaagtcct ggagatcccc ttggagccca
     3001 aaaacaacat gagggcaacc atcgactgtg cggggatctt gaagcttaga aacgccgaca
     3061 ttgagctgcg gaaaggcgag acggacattg gaagaaagaa cacgcgggtg agactggttt
     3121 tccgagttca catcccagag tccagtggca gaatcgtctc tttacagact gcatctaacc
     3181 ccatcgagtg ctcccagcga tctgctcacg agctgcccat ggttgaaaga caagacacag
     3241 acagctgcct ggtctatggc ggccagcaaa tgatcctcac ggggcagaac tttacatccg
     3301 agtccaaagt tgtgtttact gagaagacca cagatggaca gcaaatttgg gagatggaag
     3361 ccacggtgga taaggacaag agccagccca acatgctttt tgttgagatc cctgaatatc
     3421 ggaacaagca tatccgcaca cctgtaaaag tgaacttcta cgtcatcaat gggaagagaa
     3481 aacgaagtca gcctcagcac tttacctacc acccagtccc agccatcaag acggagccca
     3541 cggatgaata tgaccccact ctgatctgca gccccaccca tggaggcctg gggagccagc
     3601 cttactaccc ccagcacccg atggtggccg agtccccctc ctgcctcgtg gccaccatgg
     3661 ctccctgcca gcagttccgc acggggctct catcccctga cgcccgctac cagcaacaga
     3721 acccagcggc cgtactctac cagcggagca agagcctgag ccccagcctg ctgggctatc
     3781 agcagccggc cctcatggcc gccccgctgt cccttgcgga cgctcaccgc tctgtgctgg
     3841 tgcacgccgg ctcccagggc cagagctcag ccctgctcca cccctctccg accaaccagc
     3901 aggcctcgcc tgtgatccac tactcaccca ccaaccagca gctgcgctgc ggaagccacc
     3961 aggagttcca gcacatcatg tactgcgaga atttcgcacc aggcaccacc agacctggcc
     4021 cgcccccggt cagtcaaggt cagaggctga gcccgggttc ctaccccaca gtcattcagc
     4081 agcagaatgc cacgagccaa agagccgcca aaaacggacc cccggtcagt gaccaaaagg
     4141 aagtattacc tgcgggggtg accattaaac aggagcagaa cttggaccag acctacttgg
     4201 atgatgttaa tgaaattatc aggaaggagt tttcaggacc tcctgccaga aatcagacgt
     4261 acgtagagaa cctgtacttc cagggcgtta acggcggtgg cctgaacgat atcttcgagg
     4321 cacagaaaat cgaatggcat gaggttaact gagcgatcgc ttataagcgg ccgcgtcgag
     4381 gtcgacggta tcgataagct tgatatcgaa ttccgccccc ccccctaacg ttactggccg
     4441 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     4501 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     4561 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     4621 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     4681 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     4741 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     4801 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     4861 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     4921 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     4981 tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     5041 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtctggcgag ggcgagggcg
     5101 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     5161 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     5221 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     5281 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     5341 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     5401 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     5461 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     5521 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     5581 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     5641 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     5701 tgtacaagta agaattcctg cagcccgggg gatccgtcga cctgcagcca agcttatcga
     5761 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     5821 ggtttggcaa gctagcttaa ttaacgccat tttgcaaggc atggaaaata cataactgag
     5881 aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg
     5941 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     6001 cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     6061 ctgaaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     6121 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag
     6181 tcctccgata gactgcgtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     6241 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     6301 cagcgggggt ctttcatggg taacagtttc ttgaagttgg agaacaacat tctgagggta
     6361 ggagtcgaat attaagtaat cctgactcaa ttagccactg ttttgaatcc acatactcca
     6421 atactcctga aatagttcat tatggacagc gcagaagagc tggggagaat taattcgtaa
     6481 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6541 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6601 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6661 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6721 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6781 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6841 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6901 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6961 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7021 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7081 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7141 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7201 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7261 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7321 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7381 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7441 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7501 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7561 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7621 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7681 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7741 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7801 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7861 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7921 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7981 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8041 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8101 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8161 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8221 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8281 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8341 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8401 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8461 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8521 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8581 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8641 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     8701 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     8761 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     8821 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     8881 agagtgcacc atatgctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     8941 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     9001 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9061 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     9121 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     9181 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     9241 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     9301 catagtatat cggcatagta taatacgaca aggtgaggaa ccatatgcgg tgtgaaatac
     9361 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
     9421 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     9481 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     9541 aaacgacggc cagtgccaag ctcgctctcc cttatgcgac tcctgcatta ggaagcagcc
     9601 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
     9661 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     9721 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     9781 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga
     9841 ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt gactcaacaa
     9901 tatcaccagc tgaagcctat agagtacgag ccata
//
