LOCUS       sequence-142376-         843 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses 3XFLAG::GR LBD::eGFP from the hsp16.48 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Monsalve GC, Yamamoto KR, Ward JD
  TITLE     A New Tool for Inducible Gene Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2019 Feb;211(2):419-430. doi: 10.1534/genetics.118.301705.
            Epub 2018 Nov 30.
  PUBMED    30504365
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.118.301705"; journalName: "Genetics"; date: 
            "2019-02"; volume: "211"; issue: "2"; pages: "419-430"
COMMENT     Sequence Label: pJW1241
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..355
                     /label=hsp16-48 promoter
                     /note="promoter for the C. elegans heat shock protein 16-48
                     gene (Russnak and Candido, 1985)"
     CDS             369..434
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             441..461
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agtgacatga ttgtagtttg aagatttcac aattagagtg aatgttgttt ggttcggttt
       61 tgtcactgta tttatactca tttccacctt tttctagaac attcgagctg cttcttacaa
      121 aaggaggcga ctcacattca gaacattgag aaatagtgtg cgactgaaga aacccagata
      181 ctttttcaat ctgcgtctct ttgcacctat ggggtgtatt ttgaaatgaa tgcatctagg
      241 accttctaga acattctaaa cggctgcagg atacgggtat ataagccaat cgtgttcaga
      301 ggaaaccaat acactttgtt caagtgctta ctgttcattc tctaaacttc aagaactaga
      361 aaaaaatgga ctacaaggac cacgacggag actacaagga ccacgacatc gactacaagg
      421 acgacgacga caagatggcc ccaaagaaga agcgtaaggt cggaatccac ggagtcccag
      481 ccgccattca gcaggccact acaggagtct cacaagaaac ctctgaaaat cctggtaaca
      541 aaacaatagt tcctgcaacg ttaccacaac tcacccctac cctggtgtca ctgttggagg
      601 ttattgaacc tgaagtgtta tatgcaggat atgatagctc tgttccagac tcaacttgga
      661 ggatcatgac tacgctcaac atgttaggag ggcggcaagt gattgcagca gtgaaatggg
      721 caaaggcaat accaggtttc aggaacttac acctggatga ccaaatgacc ctactgcagt
      781 actcctggat gtttcttatg gcatttgctc tggggtggag atcatataga caatcaagtg
      841 caa
//
