LOCUS       sequence-142425-         830 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Point mutation Tyr 2105 --> Ala in the FKBP12-rapamycin binding 
            (FRB) domain of mTOR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Vilella-Bach M, Nuzzi P, Fang Y, Chen J
  TITLE     The FKBP12-rapamycin-binding domain is required for 
            FKBP12-rapamycin-associated protein kinase activity and G1 
            progression.
  JOURNAL   J Biol Chem. 1999 Feb 12. 274(7):4266-72.
  PUBMED    9933627
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Feb 12. 274(7):4266-72."
COMMENT     Sequence Label: pGEX-2T FRB Y2105A
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..306)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYAHVFRRISK"
     CDS             complement(325..342)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(369..391)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 tcagtcagtc acgatgaatt ctactggctt tgagattcgt cggaacacat gagcatagag
       61 gtcccaggct tgggtgaggt ccttgacatt ccctgatttc atgtacttcc tgcaccactc
      121 ttgggcctcc attaaatctc gaccataggc ctgattaaag gatgtttcct tcagagtctg
      181 ggggccccgt tccatcatag catgcaaggg ctccagcacc tcaaacatgc ctttcacgtt
      241 cctttcccca aagtacaaac gagatgcctc ttccaggcct tcatgccaca tctcatgcca
      301 gaggatggcc actcggatca gctcggatcc acgcggaacc agatccgatt ttggaggatg
      361 gtcgccacca ccaaacgtgg cttgccagcc ctgcaaaggc catgctatat acttgctgga
      421 tttcaagtac ttatcaattt gtgggatagc ttcaatacgt tttttaaaac aaactaattt
      481 tgggaacgca tccaggcaca ttgggtccat gtataaaaca acatcaagag cgtcatacaa
      541 catgaagtca ggatgggtta catgatcacc atttaaatat gttttatgac ataaacgatc
      601 ttcgaacatt ttcagcattt caggtagctt gctaagaaaa tcaactttga gagtttcaaa
      661 gtctttacta tatgcaattc tcgaaacacc gtatctaata tccaaaaccg ctccttcaag
      721 cattgaaatc tctgcacgct cttttggaca accacccaac atgttgtgct tgtcagctat
      781 ataacgtatg atggccatag actgtgttaa tttaacatca ccatcaatat
//
