LOCUS       sequence-142585-        9675 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Constitutive ETV1 ORF, blasticidin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9675)
  AUTHORS   Wang B, Krall EB, Aguirre AJ, Kim M, Widlund HR, Doshi MB, Sicinska 
            E, Sulahian R, Goodale A, Cowley GS, Piccioni F, Doench JG, Root DE,
            Hahn WC
  TITLE     ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to 
            MAPK Pathway Inhibition.
  JOURNAL   Cell Rep. 2017 Feb 7;18(6):1543-1557. doi: 
            10.1016/j.celrep.2017.01.031.
  PUBMED    28178529
REFERENCE   2  (bases 1 to 9675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.01"; journalName: "Cell Rep"; date: 
            "2017-02-7- 7"; volume: "18"; issue: "6"; pages: "1543-1557"
COMMENT     Sequence Label: pLX_TRC311 ETV1
FEATURES             Location/Qualifiers
     source          1..9675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2772..2796)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2804..2845
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2896..3484
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2949..2969)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3367..3378)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3556..3789
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3867..3988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3904..3923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3958..3977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4028..4163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4183..4202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4184..4202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4212..4229)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4212..4228)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4221..4243)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4370..4825
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4457..4476)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4667..4688
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4851..4955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4956..5024,5025..5816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5174..5193)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5987..6575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6476..6495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6729..6746
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6863..6884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6899..6904,6905..6922,6923..6929)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6937..6953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6942..6964
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6961..6977
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6961..6977
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6996..7016
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6998..7016
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7044..7270
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7271..7451
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7498..7623
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8116..8349
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8534..8578
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8809..9019
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8831..9027
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8878..9013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9049..9444
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9518..9635
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagcaggct
     1321 tcgaaggaga tagaaccatg gatggatttt atgaccagca agtgccttac atggtcacca
     1381 atagtcagcg tgggagaaat tgtaacgaga aaccaacaaa tgtcaggaaa agaaaattca
     1441 ttaacagaga tctggctcat gattcagaag aactctttca agatctaagt caattacagg
     1501 aaacatggct tgcagaagct caggtacctg acaatgatga gcagtttgta ccagactatc
     1561 aggctgaaag tttggctttt catggcctgc cactgaaaat caagaaagaa ccccacagtc
     1621 catgttcaga aatcagctct gcctgcagtc aagaacagcc ctttaaattc agctatggag
     1681 aaaagtgcct gtacaatgtc agtgcctatg atcagaagcc acaagtggga atgaggccct
     1741 ccaacccccc cacaccatcc agcacgccag tgtccccact gcatcatgca tctccaaact
     1801 caactcatac accgaaacct gaccgggcct tcccagctca cctccctcca tcgcagtcca
     1861 taccagatag cagctacccc atggaccaca gatttcgccg ccagctttct gaaccctgta
     1921 actcctttcc tcctttgccg acgatgccaa gggaaggacg tcctatgtac caacgccaga
     1981 tgtctgagcc aaacatcccc ttcccaccac aaggctttaa gcaggagtac cacgacccag
     2041 tgtatgaaca caacaccatg gttggcagtg cggccagcca aagctttccc cctcctctga
     2101 tgattaaaca ggaacccaga gattttgcat atgactcaga agtgcctagc tgccactcca
     2161 tttatatgag gcaagaaggc ttcctggctc atcccagcag aacagaaggc tgtatgtttg
     2221 aaaagggccc caggcagttt tatgatgaca cctgtgttgt cccagaaaaa ttcgatggag
     2281 acatcaaaca agagccagga atgtatcggg aaggacccac ataccaacgg cgaggatcac
     2341 ttcagctctg gcagtttttg gtagctcttc tggatgaccc ttcaaattct cattttattg
     2401 cctggactgg tcgaggcatg gaatttaaac tgattgagcc tgaagaggtg gcccgacgtt
     2461 ggggcattca gaaaaacagg ccagctatga actatgataa acttagccgt tcactccgct
     2521 attactatga gaaaggaatt atgcaaaagg tggctggaga gagatatgtc tacaagtttg
     2581 tgtgtgatcc agaagccctt ttctccatgg cctttccaga taatcagcgt ccactgctga
     2641 agacagacat ggaacgtcac atcaacgagg aggacacagt gcctctttct cactttgatg
     2701 agagcatggc ctacatgccg gaagggggct gctgcaaccc ccacccctac aacgaaggct
     2761 acgtgtatta aacccagctt tcttgtacaa agtggttgat atcggtaagc ctatccctaa
     2821 ccctctcctc ggtctcgatt ctacgtagta atgaactagt accggttaag tcgacaatca
     2881 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2941 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3001 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3061 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3121 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3181 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3241 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     3301 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3361 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3421 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3481 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     3541 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     3601 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3661 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3721 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     3781 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     3841 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     3901 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3961 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     4021 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4081 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4141 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     4201 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     4261 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4321 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     4381 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4441 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4501 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4561 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4621 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4681 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4741 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4801 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4861 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4921 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4981 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5041 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5101 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5161 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5221 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5281 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5341 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5401 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5461 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5521 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5581 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5641 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5701 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5761 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5821 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5881 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5941 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6001 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6061 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6121 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6601 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6661 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6721 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6781 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6841 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6901 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6961 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     7021 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     7081 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     7141 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     7201 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     7261 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7321 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7381 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7441 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     7501 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     7561 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     7621 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     7681 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     7741 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     7801 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     7861 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     7921 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     7981 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     8041 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     8101 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     8161 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     8221 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     8281 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     8341 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     8401 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     8461 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     8521 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     8581 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     8641 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     8701 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg
     8761 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     8821 atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     8881 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8941 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9001 ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccat ggccaagcct
     9061 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     9121 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     9181 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     9241 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     9301 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     9361 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     9421 ccctctggtt atgtgtggga gggcctgcag ctgcagtagt aaggcgcgcc gttaacgaat
     9481 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     9541 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     9601 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     9661 atccactttg gcgcc
//
