LOCUS       sequence-142610-         895 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     human peroxisome proliferator activated receptor alpha A/B d
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTrcHisA-PPARalpha(aa101-468)
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             30..62
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(66..74,75..89)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ggggttctca tcatcatcat catcatggta tggctagcat gactggtgga cagcaaatgg
       61 gtcgggatct gtacgacgat gacgataagg atcgatgggg atccgaatgc cgtatctgcg
      121 gtgacaaagc ttctggttac cactacggtg ttcacgcttg cgaaggttgc aaaggtttct
      181 tccgtcgtac catccgtctg aaactggttt acgacaaatg cgaccgttct tgcaaaatcc
      241 agaaaaaaaa ccgtaacaaa tgccagtact gccgtttcca caaatgcctg tctgttggta
      301 tgtctcacaa cgctatccgt ttcggtcgta tgccgcgttc tgaaaaagct aaactgaaag
      361 ctgaaatcct gacctgcgaa cacgacatcg aagactctga aaccgctgac ctgaaatctc
      421 tggctaaacg tatctacgaa gcttacctga aaaacttcaa catgaacaaa gttaaagctc
      481 gtgttatcct gtctggtaaa gcttctaaca acccgccgtt cgttatccac gacatggaaa
      541 ccctgtgcat ggctgaaaaa accctggttg ctaaactggt tgctaacggt atccagaaca
      601 aagaagctga agttcgtatc ttccactgct gccagtgcac ctctgttgaa accgttaccg
      661 aactgaccga attcgctaaa gctatcccgg gtttcgctaa cctggacctg aacgaccagg
      721 ttaccctgct gaaatacggt gtttacgaag ctatcttcgc tatgctgtct tctgttatga
      781 acaaagacgg tatgctggtt gcttacggta acggtttcat cacccgtgaa ttcctgaaat
      841 ctctgcgtaa accgttctgc gacatcatgg aaccgaaatt cgacttcgct atgaa
//
