LOCUS       sequence-142634-         905 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Monitor post-translational degradation of Aiolos AA130-189 
            (Lentiviral, 
            SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.Pur
            oR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     Artichoke
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Aiolos AA130-189 in Artichoke
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(319..321,322..834)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(361..382)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(622..641)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccagtcctcc gacagactga gtcggccggt cgaatcaagc ttatcgatac cgtcgactcc
       61 ggaatagcca ccatgtccgg attcaatgtc ttaatggttc ataagcgaag ccatactggt
      121 gaacgcccat tccagtgtaa tcagtgtggg gcatctttta ctcagaaagg taacctcctc
      181 cgccacatta aactgcacac aggggaaaaa ccttttaagt gtcacctctg caactatgca
      241 tgccaaagaa gagatgcgct cacgcgtgct gaagctgctg caaaggaagc tgcagctaag
      301 gaggctgcag ctaaggctgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      361 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      421 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      481 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
      541 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      601 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      661 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      721 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      781 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
      841 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      901 ctgcc
//
