LOCUS       sequence-142783-         839 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  GRE-dependent EGFP reporter construct flanked by the minimal Tol2 
            transposon elements.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Benato F, Colletti E, Skobo T, Moro E, Colombo L, Argenton F, Dalla 
            Valle L
  TITLE     A living biosensor model to dynamically trace glucocorticoid 
            transcriptional activity during development and adult life in 
            zebrafish.
  JOURNAL   Mol Cell Endocrinol. 2014 Jul 5;392(1-2):60-72. doi: 
            10.1016/j.mce.2014.04.015. Epub 2014 May 4.
  PUBMED    24796658
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Endocrinol."; date: "2014-07-5"; volume: "392(1-2):60-72. doi"; 
            pages: " 10.1016/j.mce.2014.04.015. Epub 2014 May 4"
COMMENT     Sequence Label: pDestTol2pA2-9xGRE
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(17..41)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        41..59
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     96..112
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     96..112
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(169..314)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     primer_bind     complement(622..638)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(623..639)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(663..682)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(664..682)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(684..704)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(724..740)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(724..740)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttgctcacca tggtccagcc tgcttttttg tacaaacttg aattaaccct cactaaaggg
       61 aacaaaagct ggagctccac cgcggtggcg gccgctctag aactagtgga tcctttacca
      121 acagtaccgg aatgccaagc tcagatctgc ggcacgctgt tgacgctgtt aagcgggtcg
      181 ctgcagggtc gctcggtgtt cgaggccaca cgcgtcacct taatatgcga agtggacctc
      241 ggaccgcgcc gccccgactg catctgcgtg ttcgaattcg ccaatgacaa gacgctgggc
      301 ggggtttgcc ggatctcgag gtcgagcggt agtgatttag ctagaacatc ctgtacagct
      361 cgacgtagct agaacatcct gtacagctcg acgtagctag aacatcctgt acagctcgac
      421 gtagctagaa catcctgtac agctcgacgt agctagaaca tcctgtacag ctcgacgtag
      481 ctagaacatc ctgtacagct cgatgtagct agaacatcct gtacagctcg acgtagctag
      541 aacatcctgt acagctcgat gtagctagaa catcctgtac agctcgacgt agctagaaca
      601 tccaatcgaa cccaagctta tcgataccgt cgacctcgag ggggggcccg gtacccaatt
      661 cgccctatag tgagtcgtat taccaacttt tctatacaaa gttgatagct tggcgtaatc
      721 atggtcatag ctgtttcctg ctcgagggcc catctggcct gtgtttcaga caccagggag
      781 tctctgctca cgtttcctgc tatttgcagc ctctctatca agactaatac acctcttcc
//
