LOCUS       sequence-142784-         827 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  GRE-dependent EGFP reporter construct flanked by the minimal Tol2 
            transposon elements.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Benato F, Colletti E, Skobo T, Moro E, Colombo L, Argenton F, Dalla 
            Valle L
  TITLE     A living biosensor model to dynamically trace glucocorticoid 
            transcriptional activity during development and adult life in 
            zebrafish.
  JOURNAL   Mol Cell Endocrinol. 2014 Jul 5;392(1-2):60-72. doi: 
            10.1016/j.mce.2014.04.015. Epub 2014 May 4.
  PUBMED    24796658
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Endocrinol."; date: "2014-07-5"; volume: "392(1-2):60-72. doi"; 
            pages: " 10.1016/j.mce.2014.04.015. Epub 2014 May 4"
COMMENT     Sequence Label: pDestTol2pA2-9xGRE
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(64..88)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    90..224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(287..307)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(315..331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    347..481
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 agggcggtgg aagatctggg aattcaaggc ctctcgagcc tctagattct gcagccctat
       61 agcacccagc tttcttgtac aaagtggggg atccagacat gataagatac attgatgagt
      121 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      181 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      241 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttccaac tttattatac
      301 atagttgata attcactggc cgtcgtttta cggtaccatc gatgatgatc cagacatgat
      361 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
      421 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
      481 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
      541 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc tagatcagat
      601 ctgcgaagat acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc
      661 gcagctgctg atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt
      721 tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact
      781 tccaaaggac caatgaacat gtctgaccaa tttcatataa tgtgaaa
//
