LOCUS       sequence-142847-         883 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses NET1A-V5 in mammalian cells in a doxycycline inducible 
            manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: FUW TetO NET1A-V5 WT
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..115)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    141..165
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttcacaaat tttgtaatcc agaggttgat tatcgataag cttgatatcg aattagctag
       61 cttaaccggt acgcgtagaa tcgagaccga ggagagggtt agggataggc ttaccttcga
      121 accgcgggcc ctctagatca accactttgt acaagaaagt tgggtacacc aaagtctctt
      181 tccgtttgcc accagaaagg gctttgtccc tcgctcttcg gatgccgggc tgtgtctggt
      241 gtgtctttaa gctcttgctg tcctctgcca tctgcatgcc agagccacat ctgtaagcgt
      301 tttcatcaac ttctacctga gtaacactgg aaactgtgga tgccctcctc tgggctgtga
      361 gtttcctcgc agaggggtgg ttcccctcac actcttcgtg cagctccggc aggccctgca
      421 gctcaggtgg actgcctgcc gactggaagg gggcaatggc cgctcgaata cagttgaacc
      481 actgctgctt gtggaacacg tcattggctt gcagagtgtg agactgggct ggagaggggt
      541 catggaagcg aattctaaag atatttttag ctttctctga gttactgaaa gctcctcgaa
      601 aggagcctcc cattctcaca tctccatcct gcaggtcttc taggactagc tcttggactg
      661 ggattggctg ccggtaaacc tggtaagagt gccgttcgtt ccgtgtgacg ggccgagtca
      721 gaaccaagat gtcttgaaac aggaaaatgt aaagtttatg tccactcttg ctccgcagct
      781 ccccatggca cagcagcact ttgctcgctt cgattctggg gtccctctgc ttttcatcca
      841 ggtactccag cttgtcgatg taatactggc actcggattc acc
//
