LOCUS       sequence-145599-         890 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Targets HA-eGFP to mitochondrial intermembrane space (IMS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pCMV0-SMAC-HA-eGFP
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      112..121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             297..323
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(330..332,333..845)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(372..393)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(633..652)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cgggccctct agaattatcg atccggaggt accatggcgg
      121 ctctgaagag ttggctgtcg cgcagcgtaa cttcattctt caggtacaga cagtgtttgt
      181 gtgttcctgt tgtggctaac tttaagaagc ggtgtttctc agaattgata agaccatggc
      241 acaaaactgt gacgattggc tttggagtaa ccctgtgtgc ggttcctatt ggatcctacc
      301 catacgatgt tccagattac gctgaattcg tgagcaaggg cgaggagctg ttcaccgggg
      361 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      421 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      481 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      541 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      601 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      661 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      721 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      781 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      841 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
//
