LOCUS       sequence-145691-         880 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral expression vector encoding untagged WT human AMPK alpha 
            2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pLenti.CMV/TO_PRKAA2
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    79..103
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgactagt cccctgtggt
       61 ggaattctgc agatatcaac aagtttgtac aaaaaagcag gcaccatggc tgagaagcag
      121 aagcacgacg ggcgggtgaa gatcggacac tacgtgctgg gcgacacgct gggcgtcggc
      181 accttcggca aagtgaagat tggagaacat caattaacag gccataaagt ggcagttaaa
      241 atcttaaata gacagaagat tcgcagttta gatgttgttg gaaaaataaa acgagaaatt
      301 caaaatctaa aactctttcg tcatcctcat attatcaaac tataccaggt gatcagcact
      361 ccaacagatt tttttatggt aatggaatat gtgtctggag gtgaattatt tgactacatc
      421 tgtaagcatg gacgggttga agagatggaa gccaggcggc tctttcagca gattctgtct
      481 gctgtggatt actgtcatag gcatatggtt gttcatcgag acctgaaacc agagaatgtc
      541 ctgttggatg cacacatgaa tgccaagata gccgatttcg gattatctaa tatgatgtca
      601 gatggtgaat ttctgagaac tagttgcgga tctccaaatt atgcagcacc tgaagtcatc
      661 tcaggcagat tgtatgcagg tcctgaagtt gatatctgga gctgtggtgt tatcttgtat
      721 gctcttcttt gtggcaccct cccatttgat gatgagcatg tacctacgtt atttaagaag
      781 atccgagggg gtgtctttta tatcccagaa tatctcaatc gttctgtcgc cactctcctg
      841 atgcatatgc tgcaggttga cccactgaaa cgagcaacta
//
