LOCUS       sequence-145801-        1029 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses PP7-2xmCherry-CAAX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Yan X, Hoek TA, Vale RD, Tanenbaum ME
  TITLE     Dynamics of Translation of Single mRNA Molecules In Vivo.
  JOURNAL   Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
  PUBMED    27153498
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-5- 5";
            volume: "165"; issue: "4"; pages: "976-89"
COMMENT     Sequence Label: pHR-PP7-2xmCherry-CAAX
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             148..852
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(290..308)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     558..577
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gatttgacca agtccctcgt cgcgacctcg caggtcgaag atcttgtcgt caaccttgtg
       61 ccgctgggcc gtcgtgcgga cccgctagcc tcctgcggcc gcggtggtgg tacgcgtgat
      121 tcgactagtg gaggaagcgg aggaggagtg agcaagggcg aggaggataa catggccatc
      181 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      241 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      301 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
      361 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
      421 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
      481 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
      541 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
      601 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
      661 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
      721 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
      781 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
      841 gagctgtaca agcatggcat ggatgagctc tacaaaggtg gaggtcggac cgaagagtac
      901 aagcttatcc tgaacggtaa aaccctgaaa ggtgaaacca ccaccgaagc tgttgacgct
      961 gctaccgcgg aaaaagtttt caaacagtac gctaacgaca acggtgttga cggtgaatgg
     1021 acctacgac
//
