LOCUS       sequence-145956-         968 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  used for expression in E. coli and purification. N-terminal GST tag 
            and C-terminal Strep tag, aa 1-389.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Schroeder CM, Ostrem JM, Hertz NT, Vale RD
  TITLE     A Ras-like domain in the light intermediate chain bridges the dynein
            motor to a cargo-binding region.
  JOURNAL   Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.
  PUBMED    25272277
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3:e03351. doi"; pages: " 
            10.7554/eLife.03351"
COMMENT     Sequence Label: pGEX6P1-human LIC1 G domain
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..92)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 ccgaaacgcg cgaggcagat cgtcagtcag tcacgatgcg gccgctcgag tcgacccggg
       61 aattctcatt tttcgaactg cgggtggctc caagttggtg gttgctttgc taaaagggac
      121 tgtagcttca taagaaacac ctgatcatct tctgccataa tttccttctc atgtacaaac
      181 tttcgaacag gtggtttagt tatgatgtct tcaaaattat cttctgcttt taatgtttga
      241 aaattttcat gtaatattcc tattttctta tcattatccc accctgctgg aataaatact
      301 gcatcctttt ccacaacaac agcaggaatc ttatagggaa atccatatag tttctgaacg
      361 atgtatttat atactaagtc tatatttttg ttttctttta ctgaagtgta aataagtgct
      421 gcaccatact gtaaacaaaa cttccggata tgtgactgaa taaaatcaaa atgttcatct
      481 ctgtagtcat gttctttctc caatacacta atggcatcac actttgtgca aactactagt
      541 actggaatgc ccaagttatg tgtaagtgta tccgcaccca gaggtaaaac tacactgtca
      601 tctttgtctt cttgtgacgc agtatttctt ctctggggag aagccgggaa gtcttctcct
      661 ggctctacat attcttggaa gtctctaatc aacttttgtt ccatttgttt catttcttca
      721 ggagggattt tcagtttgtc aacatgttct ctaacaacac ttgcccattt ctgtaaagaa
      781 tccaaagcag tccaaggctt tgacatgtca acaaccagca taactagagt atccttcaga
      841 gatacggcat ccagtgaaaa tttaaggagg cctttgtgat ataggtctcc atctaagatc
      901 caaacattac atcttgtttg atcatccctg tcttcatcat gcacatttaa gtacaaatat
      961 tccaatcc
//
