LOCUS       sequence-145958-         953 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  used for expression in E. coli and purification. N-terminal GST tag 
            and C-terminal Strep tag, aa 389-523.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Schroeder CM, Ostrem JM, Hertz NT, Vale RD
  TITLE     A Ras-like domain in the light intermediate chain bridges the dynein
            motor to a cargo-binding region.
  JOURNAL   Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.
  PUBMED    25272277
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3:e03351. doi"; pages: " 
            10.7554/eLife.03351"
COMMENT     Sequence Label: pGEX6P1-human LIC1 C-terminal half
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..40
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             458..481
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(565..587)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(732..750)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        818..922
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcctccaaaa tcggatctgg aagttctgtt ccaggggccc ctgggatcca tgactgcagc
       61 tggaaggcct gtggatgcct caccaagagt cccaggaggc tccccacgaa caccaaatag
      121 atctgtatca tctaatgttg ccagcgtgtc acccattcct gctgggtcaa aaaaaattga
      181 tccaaacatg aaagctggag ctacaagtga aggcgttctg gcaaatttct tcaacagttt
      241 gttgagtaaa aagactggct ctccaggagg ccctggtgtg agtggtggta gccctgcagg
      301 tggggctgga ggtggaagca gtggtttacc accatccacc aaaaagtcag gccagaagcc
      361 tgtcttagat gttcatgcag aactagacag aattacacga aaaccagtta cagtttctcc
      421 cacaacacct acatctccta cggaaggaga agcttcttgg agccacccgc agttcgaaaa
      481 atgagaattc ccgggtcgac tcgagcggcc gcatcgtgac tgactgacga tctgcctcgc
      541 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
      601 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      661 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt ataattcttg
      721 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      781 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      841 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      901 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc tta
//
