LOCUS       sequence-146032-        1231 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression of single-cysteine variant of single-stranded DNA-binding
            protein protein, EcSSB, as scaffold for ssDNA biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Dillingham MS, Tibbles KL, Hunter JL, Bell JC, Kowalczykowski SC, 
            Webb MR
  TITLE     Fluorescent single-stranded DNA binding protein as a probe for 
            sensitive, real-time assays of helicase activity.
  JOURNAL   Biophys J. 2008 Oct;95(7):3330-9. doi: 10.1529/biophysj.108.133512. 
            Epub 2008 Jul 3.
  PUBMED    18599625
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1529/biophysj.108.133512"; journalName: "Biophys J"; date: 
            "2008-10"; volume: "95"; issue: "7"; pages: "3330-9"
COMMENT     Sequence Label: pET22b_EcSSB_1
FEATURES             Location/Qualifiers
     source          1..1231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             644..661
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(714..732)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      728..775
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(899..918)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gggtgaacgg tacattccgt ctagaataat tttgtttaac tttaagaagg agatatacat
       61 atggccagca gaggcgtaaa caaggttatt ctcgttggta atctgggtca ggacccggaa
      121 gtacgctaca tgccaaattg tggcgcagtt gccaacatta cgctggctac ttccgaatcc
      181 tggcgtgata aagcgaccgg cgagatgaaa gaacagactg aatggcaccg cgttgtgctg
      241 ttcggcaaac tggcagaagt ggcgagcgaa tatctgcgta aaggttctca ggtttatatc
      301 gaaggtcagc tgcgtacccg taaatggacc gatcaatccg gtcaggatcg ctacaccaca
      361 gaagtcgtgg tgaacgttgg cggcaccatg cagatgctgg gtggtcgtca gggtggtggc
      421 gctccggcag gtggcaatat cggtggtggt cagccgcagg gcggttgggg tcagcctcag
      481 cagccgcagg gtggcaatca gttcagcggc ggcgcgcagt ctcgcccgca gcagtccgct
      541 ccggcagcgc cgtctaacga gccgccgatg gactttgatg atgacattcc gttctgagga
      601 tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca
      661 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      721 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      781 aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
      841 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
      901 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
      961 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaac
     1021 ttgattaggg tgatggttca cgtagtgggc atcgccctga tagacgtttt cgccctttga
     1081 cgtggagtca cgttctttat agtgactctg tcaaactgac acactcaccc tatctcggtc
     1141 tattctttga tttataggat ttgcgattcc gctatgttta gatagctgat accaagtacg
     1201 cgaattacta atttacgcct taccaattta g
//
