LOCUS       sequence-146072-         663 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Express C-terminal YN fused influenza A virus partial NS1 protein 
            (27-236 nt) for dsRNA labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Cheng X, Deng P, Cui H, Wang A
  TITLE     Visualizing double-stranded RNA distribution and dynamics in living 
            cells by dsRNA binding-dependent fluorescence complementation.
  JOURNAL   Virology. 2015 Nov;485:439-51. doi: 10.1016/j.virol.2015.08.023. 
            Epub 2015 Sep 7.
  PUBMED    26351203
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2015-11"; volume: "485:439-51. doi"; pages: " 
            10.1016/j.virol.2015.08.023. Epub 2015 Sep 7"
COMMENT     Sequence Label: pdRBFC-NS1-YN
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..72
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             77..103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    107..131
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(348..372)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(437..458)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agagctctat ttttacaaca attaccaaca acaacaaaca acaaacaaca ttacaattac
       61 atttacaatt accatgtacc catacgatgt tccagattac gctatcacaa gtttgtacaa
      121 aaaagcaggc ttcatgatgg atccaaacac tgtgtcaagc tttcaggtag attgctttct
      181 ttggcatgtc cgcaaacgag ttgcagacca agaactaggt gatgccccat tccttgatcg
      241 gcttcgccga gatcagaaat ccctaagagg aaggggcagc accctcggtc tggacatcga
      301 gacagccaca cgtgctggaa agcagatagt ggagcggatt ctgaaagacc cagctttctt
      361 gtacaaagtg gtgcctagga gtatcgctac aatggtgagc aagggcgagg agctgttcac
      421 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      481 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      541 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca
      601 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      661 cga
//
