LOCUS       sequence-146195-         794 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Express C-terminal YN fused flock house virus (FHV) B2 protein 
            (2741-2956 nt) for dsRNA labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Cheng X, Deng P, Cui H, Wang A
  TITLE     Visualizing double-stranded RNA distribution and dynamics in living 
            cells by dsRNA binding-dependent fluorescence complementation.
  JOURNAL   Virology. 2015 Nov;485:439-51. doi: 10.1016/j.virol.2015.08.023. 
            Epub 2015 Sep 7.
  PUBMED    26351203
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2015-11"; volume: "485:439-51. doi"; pages: " 
            10.1016/j.virol.2015.08.023. Epub 2015 Sep 7"
COMMENT     Sequence Label: pdRBFC-B2-YN
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(131..643,644..646,647..649))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     324..343
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     583..604
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    669..693
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aagcaaatat catgcgatca taggcgtctc gcatatctca ttaaagcagg actctaggga
       61 ctagtcccgg gtcttaatta actctctaga ctcacctaga attaactctc tagactcacc
      121 taggtcagtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct
      181 tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
      241 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
      301 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
      361 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
      421 cggggtagcg ggcgaagcac tgcaggccgt agccgaaggt ggtcacgagg gtgggccagg
      481 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
      541 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
      601 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatt gtagcgatac
      661 tcctaggcac cactttgtac aagaaagctg ggtcggcctt tccctctagg tatgccacca
      721 cgctgggttt ctccagcagt gatgttacca tccgacttac cgttagtttt gccttgttta
      781 ggcaagcgtg cagg
//
