LOCUS       sequence-146201-        3621 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Dlx4. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Dlx4
FEATURES             Location/Qualifiers
     source          1..3621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(746..764)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(747..764)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(782..798)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(782..798)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(795..817)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    806..822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(830..836,837..854,855..860))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    875..896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1013..1030)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1184..1772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1264..1283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1943..2734,2735..2803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2566..2585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2804..2908)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2986..3441)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3123..3144)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3335..3354
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3567..3589
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3581..3598
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3582..3598
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3605..3621,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3605..3621,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tgtcatttga aggagaggga gtggcaagat tgtggccgtc caaagcccct gatctaactc
      121 tctgctttgg agagccaggc ttccacacca ttctttccct ttttctcctt cggtgtcttg
      181 tcttctgaga aaatccatct ggagctggtt tcctctcctc ccagaagggg agcaggtgct
      241 cgggtcctgg gcttgacagg agggctgaag tctgaccagc ctccctccag actcacatca
      301 tctgaggcag tgccagcaca tctggggagc gatgctgata ccaggcacca aagtggctgt
      361 tgtcatagcc actggaaggc agggtgtctg ccttgggtag accccagatg aatggtaggg
      421 ctggagagtg gggagacagg gaggggggtc tcccagagaa gtcctcttcc ggctccccag
      481 agctctgttt caggagcttc ttatatttgg agcgtttgtt ctgaaaccag atctttacct
      541 gggtttgggt gagtccgagt tgtgctgcca gctgagctct ctcgggcagg gccaggtatt
      601 gggtgtgctg gaaacgctgg ttcaggtgtt ggagctgcag gctagagtag atggttctgg
      661 gcttgaatca ctagtgaatt cgcggccgcc tgcaggtcga ccatatggga gagctcccaa
      721 cgcgttggat gcatagcttg agtattctat agtgtcacct aaatagcttg gcgtaatcat
      781 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      841 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      901 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      961 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1021 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1081 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1141 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1201 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1261 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1321 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1381 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1441 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1501 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1561 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1621 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1681 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1741 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1801 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1861 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1921 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     1981 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2041 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2101 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2161 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2221 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2281 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2341 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2401 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2461 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2521 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2581 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2641 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2701 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2761 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     2821 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     2881 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgatgcgg
     2941 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     3001 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3061 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3121 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3181 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3241 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3301 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3361 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3421 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3481 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3541 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3601 attgtaatac gactcactat a
//
