LOCUS       sequence-146234-         929 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expressed sfGFP-tagged FIP3 in E. coli for protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Schroeder CM, Ostrem JM, Hertz NT, Vale RD
  TITLE     A Ras-like domain in the light intermediate chain bridges the dynein
            motor to a cargo-binding region.
  JOURNAL   Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.
  PUBMED    25272277
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3:e03351. doi"; pages: " 
            10.7554/eLife.03351"
COMMENT     Sequence Label: pET28a-FIP3
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..54)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcttccttt cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag
       61 tgcggccgct tacttgacct ccaggatgga cgggttggtc tccatgatgg ccacgatgat
      121 cctgtcgatg tagtcctgca ggcggaagtt gatctcctcc tgcttctgaa tcgcctccat
      181 gagctcatct cgggagacgg agctgatctc tgcagccagg gactcagaga aggctgtgga
      241 gaagaggctc ttggcgccct ggatgctgag ggtaatgatc tgcccgttca gctcctcgtt
      301 ctgctccttc aggttgcggt tgtcctgctt cagcctgcgg acctcctgct ccagctcgct
      361 ctcccgggcg cggctgtggt actcctgcag gcccatgctg ctgctgcggc cccgccgctg
      421 ctcggcctcc agcttgagga gctgcaggtg ctccagctgc tttcggaggt cctcgatcag
      481 ctcctgggtg gcctccttgt ccctctggaa ctggtgcctc tcgtgactca gcctgtcccc
      541 cattctcctc ttgttctcct gctcttcact gagccgcagc gtcagcgact ctatctcatc
      601 caacagcttc tgcttctcct cctccagacg ctcaatgttg gccttcagac agggcgtgca
      661 ggaccggagt tcactgttct cctcgtccag ttgctgtagc ctggtctgca ggttctcgat
      721 ctcaatgctc ttctccctct ccatcttgca caggagctcc ttctgacgcc gggtctcttc
      781 caggaccatc tcgcaggctc tcagctcctg ctccttcagc tgctcctcca gggcgtttgc
      841 tctgtgcacc agctgcaggt tctcctgcct caggcggctg tgttgctcac cggtggctgc
      901 cgtgtccttt tccagctcca gcacacgcc
//
