LOCUS       sequence-146356-        3597 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Dlx5. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Dlx5
FEATURES             Location/Qualifiers
     source          1..3597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(722..740)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(723..740)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(758..774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(758..774)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(771..793)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    782..798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(806..812,813..830,831..836))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    851..872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(989..1006)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1160..1748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1240..1259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1919..2710,2711..2779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2542..2561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2780..2884)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2962..3417)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3099..3120)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3311..3330
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3543..3565
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3557..3574
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3558..3574
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3581..3597,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3581..3597,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tattgatagt gtccacagtt gcgcaaaaaa aaaaaaaaaa gggttctttt cgtaaaaaag
      121 ggggcggggc tctctgaaat gcaataactt acatgcaaaa aaaagtttac acatgaatct
      181 tttttgtttc cattgttcaa acatccccgt atgaattcct ttctttctga tttctaaacc
      241 agcacaacac tgtagtccca aaactgagca agagaaagta gcccatctaa taaagcgtcc
      301 cggaggccag tgccagcggg tgctgcaagg agcccggcgg tggcaggtgg gaattgattg
      361 agctggctgc gcttgggtac caggaagccg agttctccag gtagctggac gcgggggact
      421 ggttggaggt cggagggtgg gcatgagggt ggtggctgag agagcgggat gagccctggg
      481 gctcccacac cgctggtgac tgtggcgagt tacacgccat agggtcgctg gagctgggac
      541 tgtgctccgg gggcatctcc ccgtttttca tgatcttctt gatcttggat cttttgttct
      601 gaaaccagat tttcacctgt gtttgcgtca gtcctagaga gaatcactag tgaattcgcg
      661 gccgcctgca ggtcgaccat atgggagagc tcccaacgcg ttggatgcat agcttgagta
      721 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      781 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      841 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      901 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      961 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1021 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1081 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1141 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1201 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1261 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1321 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1381 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1441 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1501 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1561 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1621 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1681 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1741 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1801 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1861 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1921 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1981 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2041 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2101 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2161 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2221 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2281 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2341 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2401 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2461 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2521 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2581 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2641 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2701 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2761 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2821 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2881 cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta
     2941 aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     3001 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     3061 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     3121 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     3181 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     3241 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     3301 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     3361 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca
     3421 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     3481 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     3541 ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactata
//
