LOCUS       sequence-146446-        1977 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  protein expression of chicken DCTN1 p150Glued AA 217-548 (CC1) in 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Quintyne NJ, Gill SR, Eckley DM, Crego CL, Compton DA, Schroer TA
  TITLE     Dynactin is required for microtubule anchoring at centrosomes.
  JOURNAL   J Cell Biol. 1999 Oct 18;147(2):321-34.
  PUBMED    10525538
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "1999-10-18"; volume: "147(2)"; pages: "321-34"
COMMENT     Sequence Label: pVEX-CC1
FEATURES             Location/Qualifiers
     source          1..1977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1102..1120)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1116..1163
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(1529..1534,1535..1551,1552..1557))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(1584..1602)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1670..1774
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttccctcta gaaataattt tgtttaactt taagaaggga gatatacata tggaggaaga
       61 aaatctgcgt tcccaagtca gggacctgga ggaaaagctg gagacgctga agatcaagcg
      121 caatgaagac aaagccaagc ttaaggagct ggagaagtac aagatccagc tggagcaggt
      181 gcaggagtgg aagagcaaga tgcaggagca gcaggcagac ctgcagagac ggttaaagga
      241 ggccaagaag gaagccaaag atgctctgga agccaaggag cgctacatgg aggagatggc
      301 cgacactgcc gacgccatcg agatggcaac cctggacaag gagatggcgg aggagcgggc
      361 ggagtccctg cagcaggagg tggactccct gaaggagaag gtggagtacc tcaccatgga
      421 cctggagatc ctgaagcacg agatcgagga gaaaggctcg gatggagcgg catccagtta
      481 ccaggtcaag cagctggagg agcaaaatgc gaggctgaag gaagctcttg tgaggatgag
      541 ggacctctcc gcgtccgaga agcaggaaca cgtgaagctc cagaagcaaa tggagaagaa
      601 aaacacggag ctggaatcgc tgcggcagca gagggagaaa ctgcaggagg aggtgaagca
      661 ggcggagaaa acggtcgatg agctgaagga gcaggtggac gccgctctgg gtgccgaaga
      721 gatggtggag actctgacag agagaaacct ggacctggag gagaaggtcc gggagctgcg
      781 cgagaccgtc ggggacctgg aagccatgaa tgagatgaac gatgagctgc aggagaacgc
      841 ccgggagacg gagctggagc tgcgggagca gctggacctg gcggcggcgc gcgtgcggga
      901 ggcggagaag cgcgtggagg ccgcccagga gaccgtggcc gactaccagc agaccatcaa
      961 gaagtaccgc gagctgacgg cgcatctgca ggacgtgaac cgggagctca tgagtcagca
     1021 ggaggcgtct gcagagaagc agcagcagta aggatccggc tgctaacaaa gcccgaaagg
     1081 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     1141 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg
     1201 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
     1261 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc
     1321 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
     1381 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
     1441 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
     1501 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca
     1561 tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     1621 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1681 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1741 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1801 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1861 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1921 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgag
//
