LOCUS       sequence-146672-         339 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Retroviral vector with ubiquitin variant that binds to and occludes 
            the ligand binding site of the 53BP1 Tudor domain (i53).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Canny MD, Moatti N, Wan LCK, Fradet-Turcotte A, Krasner D, 
            Mateos-Gomez PA, Zimmermann M, Orthwein A, Juang YC, Zhang W, 
            Noordermeer SM, Seclen E, Wilson MD, Vorobyov A, Munro M, Ernst A, 
            Ng TF, Cho T, Cannon PM, Sidhu SS, Sicheri F, Durocher D
  TITLE     Inhibition of 53BP1 favors homology-dependent DNA repair and 
            increases CRISPR-Cas9 genome-editing efficiency.
  JOURNAL   Nat Biotechnol. 2018 Jan;36(1):95-102. doi: 10.1038/nbt.4021. Epub 
            2017 Nov 27.
  PUBMED    29176614
REFERENCE   2  (bases 1 to 339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.4021"; 
            journalName: "Nat Biotechnol"; date: "2018-01"; volume: "36"; issue:
            "1"; pages: "95-102"
COMMENT     Sequence Label: pMX-HA-UbvG08-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..339
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..67
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tctagactgc cggatctagc tagttaatta agccaccatg tacccatacg atgttccaga
       61 ttacgctgca gctgcaatgt tgattttcgt gaaaaccctt accgggaaaa ccatcaccct
      121 cgaggttgaa ccctcggata cgatagaaaa tgtaaaggcc aagatccagg ataaggaagg
      181 aattcctcct gatcagcaga gactggcctt tgctggcaaa tcgctggaag atggacgtac
      241 tttgtctgac tacaatattc taaaggactc taaacttcat cctctgttga gacttcgttg
      301 agcggccgct acgtaaattc cgcccccccc ccccctctc
//
