LOCUS       sequence-146824-         818 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  luciferase reporter for miRNA activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Eichelser C, Stuckrath I, Muller V, Milde-Langosch K, Wikman H, 
            Pantel K, Schwarzenbach H
  TITLE     Increased serum levels of circulating exosomal microRNA-373 in 
            receptor-negative breast cancer patients.
  JOURNAL   Oncotarget. 2014 Oct 30;5(20):9650-63.
  PUBMED    25333260
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Oncotarget."; date: "2014-10-30"; volume: "5(20)"; pages: "9650-63"
COMMENT     Sequence Label: 625_HOXD13 in pmiRGlo
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(233..251)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    288..409
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(299..318)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     353..372
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(677..697)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aaggactgac cggcaagttg gacgcccgca agatccgcga gattctcatt aaggccaaga
       61 agggcggcaa gatcgccgtg taattctagt tgtttaaacg agctcgctag cggaaagact
      121 tgaatatgta tttaattccc cccaccccct gccatctaga gtcgacctgc aggcatgcaa
      181 gctgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
      241 caataactag cataacccct tggggcggcc gcttcgagca gacatgataa gatacattga
      301 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
      361 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
      421 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt taagcaagta
      481 aaacctctac aaatgtggta aaatcgaatt ttaacaaaat attaacgctt acaatttcct
      541 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gcggatctgc
      601 gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc
      661 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
      721 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
      781 ccaggctccc cagcaggcag aagtatgcaa agcatgca
//
