LOCUS       sequence-146832-         839 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  luciferase reporter for miRNA activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Eichelser C, Stuckrath I, Muller V, Milde-Langosch K, Wikman H, 
            Pantel K, Schwarzenbach H
  TITLE     Increased serum levels of circulating exosomal microRNA-373 in 
            receptor-negative breast cancer patients.
  JOURNAL   Oncotarget. 2014 Oct 30;5(20):9650-63.
  PUBMED    25333260
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Oncotarget."; date: "2014-10-30"; volume: "5(20)"; pages: "9650-63"
COMMENT     Sequence Label: 766_SMAD4 in pmiRGlo
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(227..245)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    282..403
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(293..312)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     347..366
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     enhancer        648..839
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(671..691)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tgaccggcaa gttggacgcc cgcaagatcc gcgagattct cattaaggcc aagaagggcg
       61 gcaagatcgc cgtgtaattc tagttgttta aacgagctcg ctagctcttt cagatgtgtt
      121 tacttttgcc tggagaactt ttagctattc tagagtcgac ctgcaggcat gcaagctgat
      181 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      241 ctagcataac cccttggggc ggccgcttcg agcagacatg ataagataca ttgatgagtt
      301 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
      361 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      421 tcattttatg tttcaggttc agggggagat gtgggaggtt tttttaagca agtaaaacct
      481 ctacaaatgt ggtaaaatcg aattttaaca aaatattaac gcttacaatt tcctgatgcg
      541 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca
      601 ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag
      661 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
      721 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
      781 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtccc
//
