LOCUS       sequence-146964-         634 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Determine its activity and specificity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Fahie K, Hu P, Swatkoski S, Cotter RJ, Zhang Y, Wolberger C
  TITLE     Side chain specificity of ADP-ribosylation by a sirtuin.
  JOURNAL   FEBS J. 2009 Dec;276(23):7159-76. doi: 
            10.1111/j.1742-4658.2009.07427.x. Epub 2009 Nov 6.
  PUBMED    19895577
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1742-4658.2009.07427.x"; journalName: "FEBS J"; date: 
            "2009-12"; volume: "276"; issue: "23"; pages: "7159-76"
COMMENT     Sequence Label: TbSIR2RP1
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..66)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(67..84)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aggatccgaa ttctacatca ccactttgta caagtaaagc tgggtctagt ggtggtggtg
       61 gtggtgagaa ccccggggaa ctaaaccctc aacgactttt tccctacgcc gagtgcgctc
      121 aaatatttcc cttccctcac gtacagaggc gtcaagctgc tctccgaagc ccaacgcctg
      181 ggcaaaggaa cgaacagaca cctgacagtc accaggaaag aaaatgtcac gcaacacgcc
      241 cgtctcgtca ttctctgcga gcgtaaactg gtcatacccg tcgacgctgc tgctgttgct
      301 gctgtcccca ctgctaattt tactgccatt gccatttcca gtgctgagac gataggaact
      361 ggcgcggaaa ttgggtgttc ggtccgtagg aaagcggaac atcctcccgc ccacgcgctc
      421 caagttaaac aacactcgtg gcacgtcaga gggcaccatg agcgcaaggt cggcaaatgg
      481 gtggacttna agtgaggttc ccaaaataag cagcaattcc gtttcctcaa tgagtcccgc
      541 tacgttaaaa aacgcctccg ggagattctc gccaaagaaa accacgtcgg gtttcactat
      601 accaccacat tgattgcaat gaggcacttt tcca
//
