LOCUS       sequence-147028-         832 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  An AAV packaging vector that expresses eGFP and Kv1.2 under the 
            control of a neuronal promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Kourrich et al.
  TITLE     AAV Potassium channel
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     207..228
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      270..279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(299..746)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa
       61 gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
      121 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
      181 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
      241 cccggtgaac agctcctcgc ccttgctcac catggtggcg gatccggtac cttctcgact
      301 gcgctctcag gcacgacacg actcctccgc tgcccaccgc agactgaggc agcgctgagt
      361 cgccggcgcc gcagcgcaga tggtcgcgcc cgtgcccccc tatctcgcgc ctcgcgtggt
      421 gcggtccggc tgggccggcg gcggcgcgga cgcgaccaag gtggccggga aggggagttt
      481 gcgggggacc ggcgagtgac gtcagcgcgc cttcagtgct gaggcggcgg tggcgcgcgc
      541 cgccaggcgg gggcgaaggc actgtccgcg gtgctgaagc tggcagtgcg cacgcgcctc
      601 gccgcatcct gtttcccctc cccctctctg ataggggatg cgcaatttgg ggaatggggg
      661 ttgggtgctt gtccagtggg tcggggtcgg tcgtcaggta ggcaccccca ccccgcctca
      721 tcctggtcct aaaacccact tgcactcata cgcagggccc tctgcagtct agacacacgc
      781 gtgcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cg
//
