LOCUS       sequence-147032-        1009 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Photinus pyralis sulfotransferase ST3 - pHis8-4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Fallon TR, Li FS, Vicent MA, Weng JK
  TITLE     Sulfoluciferin is biosynthesized by a specialized luciferin 
            sulfotransferase in fireflies.
  JOURNAL   Biochemistry. 2016 May 26.
  PUBMED    27227579
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2016 May 26."
COMMENT     Sequence Label: pJKW 0690
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     285..304
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     388..407
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        388..406
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    407..431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             446..468
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             481..504
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             511..531
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQA"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      121 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
      181 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
      241 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      301 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      361 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
      421 ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgaaa
      481 caccaccacc accaccacca ccacggtggt gaaaacttgt acttccaggc ccatggcatg
      541 ccacataaca ttcaaattgg ggatatttta aataatagag atgaaaatag taagaggaaa
      601 tttccttacg aagttaaaat tgtagatcct gacacaaata aggagctatt gaatgttttt
      661 acaggggagc atacagggtt cgtacaagta ggccctcaaa aatatttctt tcctagcggc
      721 tatgaaaagg tagcagaaaa atattacaat tttgaaatac gacccgacga tgtctggatt
      781 gttacatttc caagatcagg tacaacgtgg acgcaagaaa tggtgtggtt aattgcaaac
      841 gaatttgact acaatacggc cgctagagta ccattaaacg aaagatttcc ttttttagaa
      901 ttcagcatgt gcgtccatcc gaaaatgaag gcacaattct tacaagaaaa tagtcacagt
      961 attgaacgac aaaatatgat cgaagacgtc tgcacagaag catggaaga
//
