LOCUS       sequence-147077-        8437 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Modular vector for constitutive secreted amylase reporter 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Oliveira Paiva AM, Friggen AH, Hossein-Javaheri S, Smits WK
  TITLE     The Signal Sequence of the Abundant Extracellular Metalloprotease 
            PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in 
            Clostridium difficile.
  JOURNAL   ACS Synth Biol. 2016 Jun 23.
  PUBMED    27333161
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Jun 23."
COMMENT     Sequence Label: pSJ111
FEATURES             Location/Qualifiers
     source          1..8437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2402..2773)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            2806..2915
                     /note="incP origin of transfer"
     primer_bind     complement(4104..4121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4275..4863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4355..4374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccttattaac gttgatataa tttaaatttt atttgacaaa aatgggctcg tgttgtacaa
       61 taaatgtaga gctcctgcag taaaggagaa aattttatga gaccaagtaa aaaattatta
      121 atagctataa tatcaatatt cttaataagt tcagttccag taagtgcaca tgcagatagt
      181 gcaaacaaaa gtaatgaatt aacagcacct agtatcaaaa gtggaacaat tctacatgct
      241 tggaactggt catttaacac tttgaaacac aatatgaaag atatacatga tgctggatat
      301 acagcaatac agacttctcc aataaaccag gtaaaagagg gaaatcaagg ggataaatca
      361 atgtctaatt ggtattggtt atatcaacca acatcatatc aaataggaaa taggtatctt
      421 ggtactgaac aagagtttaa agaaatgtgt gctgcagctg aagaatatgg aataaaggtt
      481 attgtagatg ctgtcataaa tcatacaact tcagactatg ctgctatatc taacgaagta
      541 aagtctattc caaattggac acatggtaat acacagatta agaattggtc tgatcgatgg
      601 gatgtgacac aaaactcttt actaggtctt tatgattgga atactcaaaa tactcaagta
      661 caatcatatc ttaaaagatt tttggacagg gctcttaacg atggagcaga tggttttaga
      721 ttcgatgcag caaaacacat agaattgcca gacgatggat catatggtag tcagttttgg
      781 ccaaacataa caaatactag tgctgaattt caatatggtg agattttaca agattctgca
      841 agtagagatg cagcatatgc gaactatatg gatgttactg ctagtaatta cggtcatagt
      901 attagatcag ctctaaagaa taggaattta ggagtttcta atatctctca ttatgcctct
      961 gatgtttcag cagataaact agtaacttgg gttgaaagcc atgatacata tgcaaacgac
     1021 gatgaagaat ctacatggat gtctgatgat gatataagat taggttgggc agttatagca
     1081 tctagaagtg gttctacacc attattcttt tcaagacctg aaggtggagg aaatggagtg
     1141 cgatttccag gaaaaagtca aattggtgat agaggttcag ccttatttga ggaccaagca
     1201 attactgctg taaatagatt tcataatgta atggcagggc aacctgaaga attaagtaat
     1261 ccaaatggga acaatcaaat ctttatgaat caacgtggta gtcacggagt agttttagca
     1321 aatgcaggaa gttcatccgt ttcaataaac actgcaacaa agttacctga tggtagatat
     1381 gacaataaag caggagcagg gagttttcaa gtaaatgatg ggaaactaac aggtacaatt
     1441 aacgcaagat cagtagctgt tctatatcct gatgatatcg caaaagcccc acatgtattc
     1501 ttggagaatt acaaaacagg tgtgacgcat tcctttaatg atcaattaac tataacatta
     1561 agagctgatg ctaatacaac taaagctgtc taccaaatca ataatggtcc agatgataga
     1621 agattgagaa tggagattaa cagtcaatca gaaaaagaaa ttcaatttgg taaaacatat
     1681 acaataatgt taaagggaac taactctgat ggagtaacaa ggacggaaaa gtattctttt
     1741 gtcaaaagag atccagcttc agctaagacg ataggatatc agaaccctaa tcattggtca
     1801 caagtgaatg cttatattta taaacatgat ggatctagag ttatagaatt aaccggatca
     1861 tggccaggta aacctatgac taaaaacgct gatggaatat acactttaac actaccagca
     1921 gacacagata ctactaatgc taaagtgata ttcaataacg gaagtgcaca agtacctggt
     1981 cagaaccaac ctggttttga ctacgtactt aatggattat acaatgatag cggattatct
     2041 ggaagtttac cacattaagg atcctataag ttttaataaa actttaaata gaaaaaggct
     2101 tctctcatga gaagtctttt ttatttaaaa taaatataaa ataaaataga ggctataaat
     2161 agcctctatt ttatgtgaga aatccctaaa taaaaagatg ccagtgtgct ggaattcgcc
     2221 cttaggctgc gctagggacc tctttagctc cttggaagct gtcagtagta ggggatcggt
     2281 cttgccttgc tcgtcggtga tgtacttcac cagctccgcg aagtcgctct tcttgatgga
     2341 gcgcatgggg acgtgcttgg caatcacgcg caccccccgg ccgttttagc ggctaaaaaa
     2401 gtcatggctc tgccctcggg cggaccacgc ccatcatgac cttgccaagc tcgtcctgct
     2461 tctcttcgat cttcgccagc agggcgagga tcgtggcatc accgaaccgc gccgtgcgcg
     2521 ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg ccattgatgc
     2581 gggccagctc gcggacgtgc tcatagtcca cgacgcccgt gattttgtag ccctggccga
     2641 cggccagcag gtaggccgac aggctcatgc cggccgccgc cgccttttcc tcaatcgctc
     2701 ttcgttcgtc tggaaggcag tacaccttga taggtgggct gcccttcctg gttggcttgg
     2761 tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca
     2821 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
     2881 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
     2941 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
     3001 ccgaaaaaat cgctataatg accccgaagc aggaagggcg aattctgcag atctgaccgg
     3061 tctctgaaaa tataaaaacc acagattgat actaaaacct tggttgtgtt gcttttcggg
     3121 gcttaaatca aggaaaaatc cttgttttaa gcctttcaaa aagaaacaca aggtctttgt
     3181 actaacctgt ggttatgtat aaaattgtag attttagggt aacaaaaaac accgtatttc
     3241 tacgatgttt ttgcttaaat acttgttttt agttacagac aaacctgaag ttaactattt
     3301 atcaattcct gcaattcgtt tacaaaacgg caaatgtgaa atccgtcaca tactgcgtga
     3361 tgaacttgaa ttgccaaagg aagtataatt ttgttatctt ctttataata tttccccata
     3421 gtaaaaatag gaatcaaata atcatatcct ttctgcaaat tcagattaaa gccatcgaag
     3481 gttgaccacg gtatcataga tacattaaaa atgttttccg gagcatttgg ctttccttcc
     3541 attctatgat tgtttccata ccgttgcgta tcactttcat aatctgctaa aaatgattta
     3601 aagtcagact tacactcagt ccaaaggctg gaaaatgttt cagtatcatt gtgaaatatt
     3661 gtatagcttg gtatcatctc atcatatatc cccaattcac catcttgatt gattgccgtc
     3721 ctaaactctg aatggcggtt tacaatcatt gcaatataat aaagcattgc aggatatagt
     3781 ttcattccct tttcctttat ttgtgtgata tccactttaa cggtcatgct gtaggtacaa
     3841 ggtacacttg caaagtagtg gtcaaaatac tcttttctgt tccaactatt tttatcaatt
     3901 ttttcaaata ccatctaagt tccctctcaa attcaagttt atcgctctaa tgaacaaaga
     3961 tattatacca catttttgtg aatttttcaa cttgcccact tcgactgcac tcccgactta
     4021 ataacttctt gaacacttgc cgaaaaagaa aaactgccgg gtacgtaccc gggatcgatc
     4081 cccgccgagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccactga atcaggggat
     4201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4261 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4441 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatca cagttcggtg
     4501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4681 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     4741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4921 taagggattt tggtcatgaa atgcaagttt ctaactaaca tacatcatag ttactaaact
     4981 atggtaacta taattttatt aactatatta agcactgatt agtactataa ctcaatataa
     5041 gcatatcccc tgtatcgtaa ctagagaacc aaacgacgga aaaagcgata tagataagtt
     5101 tagatagtgc accaattttt tacttttcta ttttagaatc tagcatttcc aatgcttact
     5161 aatactattt ttgattttta acttgtttta aaaaagcacc tactaattag gattctagct
     5221 ctatattcta gttatacatt ctctatattc tagctctata ttctagttat acattctcta
     5281 tattctagct ctatattcta gttatacatt ctctatattc tagctctata ttctagttat
     5341 acattctcta tattctagct ctatattcta gttatacatt ctctatattc tagctctata
     5401 ttctagttat acattctcta tattctagct ctatattcta gttatacatt ctctaaaaaa
     5461 catatatata tatactacat atatatgtac aattatttta ccaaaagaaa tttttaaaac
     5521 ggcttaattt caatactttg atgccatttt ttttttgcaa ttctcgtaca attattttac
     5581 caaaagaaat ttttattaat tagttttttt gcataaaaat aggactagaa aatcaattct
     5641 aatcctatca aaaatctact tgtttaatct atatgtatag tacataaatc ctctatcatt
     5701 tattttagtt tcccatacat ctaaaatttt agtatctaat aaagattcta tcatatttga
     5761 aattctttgc tgtgttactc ctaaatcttt tgctaattcc tcttgattaa ttcttaatat
     5821 atttcctttt attttatcat ctttgaccaa taaatcttgc ttatatctca tatgagtata
     5881 taatcttaat tcttcagttg aaattattcc ccaaattact gccatggtaa caaagtaact
     5941 aatagtatat tgtttatcta tattacatct aacttctttt acattgtatg tatcttttat
     6001 tcctagcttt gtaacacctt ttgtttttgt aatataatca ttatgttgaa gttcttttaa
     6061 tgtttctctt aatgtctttt cactcataac aatgttttta actttcttct tacgtttata
     6121 ggttatcagc tccattatat cgtctatgtt taattctcta tctttattgt taagtaacac
     6181 attatagata aacaattgat taccagtcat tattttaacc cctttcctat tcttatattt
     6241 caaatccttt gagtgcttat gtggcatatt gaacaaagta tcttcatcac tatatataaa
     6301 atctgattct attttattcc aacaatcact attaaactca caacctgtac aaccaaaatt
     6361 aaccgtttta agcttctcat acatatactt gacttgatat tctagctcac tatttgaaag
     6421 actattttgt gatatatagt tccattctct agcaacggat attacttgac ttaaactttt
     6481 attcctcaat cttaaagtca caactatttt ttgcaaatct aggtttttat gtccatcttt
     6541 actaccattt tttaaaatat ctactataca atttggtagc ttactatcta atatatattt
     6601 tgtattagta ttacctactg tttttacatc tttgcaatat tttttagcta acttttctat
     6661 gtcatatcta tatatatttt tgtcttggtg tattattttt acttgtttag tagtattttt
     6721 aatattatat gtataaggta ctcttaatac ttgagtagat aaatttgctt gtttatctgc
     6781 acccaattta tttattaatg tatttgtaac ttctgatact aacttaatgt tattagtttt
     6841 attaattagc acataaacat ggaatccatt tccactatca actaggatat gataatgtaa
     6901 tcctatctta gtaaataaat taattatatc tttataatta aaaccttgtc ctaattcttt
     6961 tttgtcaaaa tcaaatccta atgcatatct atttattaag ttttcattgg ttcctgcttt
     7021 tccatcagtt gtacataggt taaaaaatgt attttcataa taaatatttt ttgcgaaggt
     7081 aaattctact atatcatcta tatttttaaa aaaagttgtt ttactatatt ctttaaattc
     7141 ttctccgagt tctaattgtt tactattact tttattattt tgaaatactc taacatattc
     7201 attatcaata agattatttc cttgtcctat accatctcta aatactgcca ttagaaattt
     7261 tttcaactta tattttgaat ctaattgttc catatattta ttatctcctt atctagtttt
     7321 tttaagagat aacactacat taaatatata cttattgact aatcaacttt tacaactatt
     7381 gaataatttt agcttagtat gtataataat attgttaaga tgctaaaatt gatagcaata
     7441 ttattatata actacagata cttaatcagt tagaccgata ttggagttgg tagctccata
     7501 aactcaattg gtagttgagt tgtcattgtc tagtagtatt atctcatttt gttgttatct
     7561 taaaatatta ttttaaaaat actctgcttg gtcggcttag ggtatttttt ttgtttaatt
     7621 ccaatattgg ttcaaactcc tgtgtaatac ttttgacact tccatctgta ttgaattgac
     7681 agttgatatg gtatctcatt attggcttgt tcttatgttt ttctatgtat ttatttttta
     7741 ctttcaaata atttgtattt ccatttttag ttatgtttaa cacactatcc ttattactca
     7801 ataaaattat tttttgtttt aacatatgat ttatagaatc taaaaattga tattgactta
     7861 taatagtact ttctacctct ctagcttttt ctagtgtttc aaattcttca aactctgcta
     7921 tattgctata gtatagctta tcattaataa tattatctga caactttaat tccattttat
     7981 aaaacaattc caagttatgt ataaaatttt taatgttaca acatatagac ttattatttg
     8041 ttcttatatg gtatctccat ttactacttc tagctctcat gttatatgca caactactcc
     8101 caacataaac atatattgct tgttctaact catatttttg taaaatttca acatcatatc
     8161 cagtcaaatc gtactctctc ttatatacaa atggtttttc taattttttg ttcaataaat
     8221 ctcctcctat ttcttactta atttagctaa aaatctattt atatttcacc tcctttttga
     8281 ctttaagcct acgaatacca tatttatgat tataacatct tctatttttc ccaaatcctt
     8341 acatctcccc taatttttag acttaagggc gaattctgca tcaagctagc ataaaaataa
     8401 gaagcctgca tttgcaggct tcttattttt atggtac
//
