LOCUS       sequence-147092-         624 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral over-expression vector for YAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Qin H, Hejna M, Liu Y, Percharde M, Wossidlo M, Blouin L, 
            Durruthy-Durruthy J, Wong P, Qi Z, Yu J, Qi LS, Sebastiano V, Song 
            JS, Ramalho-Santos M
  TITLE     YAP Induces Human Naive Pluripotency.
  JOURNAL   Cell Rep. 2016 Mar 15;14(10):2301-12. doi: 
            10.1016/j.celrep.2016.02.036. Epub 2016 Mar 3.
  PUBMED    26947063
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.02.036"; journalName: "Cell Rep"; date: 
            "2016-03-15- 15"; volume: "14"; issue: "10"; pages: "2301-12"
COMMENT     Sequence Label: pGAMA-YAP
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(108..161)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(165..206)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    232..256
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg
       61 atggccatgt tatcctcctc gcccttgctc accatggtgg cgaccggtgg gccaggattc
      121 tcctcgacgt caccgcatgt tagcagactt cctctgccct cacgcgtaga atcgagaccg
      181 aggagagggt tagggatagg cttaccttcg aaccgcgggc cctctagatc aaccactttg
      241 tacaagaaag ctgggtcggc gcgcccaccc tttaaccatg taagaaagct ttctttatct
      301 agcttggtgg cagccaaaac agactccatg tcattaagga tgtcagaact caaagcttcc
      361 tgcagacttg gcatcagctc ctctccttct atgttcattc catctccttc cagtgttcca
      421 aggtccacat ttgtcccagg aatggcttca aggtagtctg ggaaacggtt ctgctgtgag
      481 ggcagggtgc tttggttgat agtatcacct gtatccatct catccacact gttcaggaag
      541 tcatctgggg ttcgagggac actgtagctg ctcatgctta gtccactgtc tgtactctca
      601 tctcgagagt gataggtgcc actg
//
