LOCUS       sequence-147150-         677 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Membrane, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-UbiquitinC-C-18
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(142..366)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(421..672)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(463..484)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ataacagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
       61 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtta
      121 tctagatccg gtggatcctc acccacctct gagacggagt accaggtgca gggtggactc
      181 tttctggatg ttgtagtcag acagggtgcg cccatcttcc agctgttttc cagcaaagat
      241 cagcctctgc tggtcaggag ggatgccttc cttatcttgg atctttgcct tgacattctc
      301 aatggtgtca ctgggctcaa cctcgagggt gatggtctta ccagtcagag tcttcacgaa
      361 gatctgaccg ctctccaccg ctgccgaatt cgaagcttga gctcgagatc tgagtccgga
      421 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
      481 catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactggtagc tcaggtagtg
      541 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      601 gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg aagttggcct tgatgccgtt
      661 cttctgcttg tcggcgg
//
