LOCUS       sequence-147212-         862 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses biosensor for cAMP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Odaka H, Arai S, Inoue T, Kitaguchi T
  TITLE     Genetically-encoded yellow fluorescent cAMP indicator with an 
            expanded dynamic range for dual-color imaging.
  JOURNAL   PLoS One. 2014 Jun 24;9(6):e100252. doi: 
            10.1371/journal.pone.0100252. eCollection 2014.
  PUBMED    24959857
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-06-24"; volume: "9(6):e100252. doi"; pages: " 
            10.1371/journal.pone.0100252. eCollection 2014"
COMMENT     Sequence Label: Flamindo2
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(215..236)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(476..495)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacgggccc tctagactcg agcggccgcc
      121 actgtgctgg atatctgcag aattccacca cactggacta gtggatccca tggtgagcaa
      181 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      241 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      301 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      361 cttcggctac ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
      421 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      481 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      541 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      601 caacctccgc ggggcactga agaaagagga gcttgttgag gctatggccc tcctgtccca
      661 gcgagggcct gatgccctac tcaccgtggc gctccggaag cccccaggtc agcgtacgga
      721 tgaagaactg gacctgatct ttgaagagct gctgcatatc aaggcggtgg cacacctttc
      781 taactcggtg aagcgggaac tagctgctgt tctgctcttt gaaccacaca gcaaggcagg
      841 aactgtgttg ttcagccagg gg
//
