LOCUS       sequence-147328-         695 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses the acyl carrier protein (ACP) from the E. coli fatty acid
            synthase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Worthington AS, Rivera H, Torpey JW, Alexander MD, Burkart MD
  TITLE     Mechanism-based protein cross-linking probes to investigate carrier 
            protein-mediated biosynthesis.
  JOURNAL   ACS Chem Biol. 2006 Dec 20;1(11):687-91.
  PUBMED    17184132
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol."; date: "2006-12-20"; volume: "1(11)"; pages: "687-91"
COMMENT     Sequence Label: AcpP pet29a C-terminal His Tag
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..70
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             86..103
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             122..352
                     /codon_start=1
                     /gene="acpP"
                     /product="acyl carrier protein (ACP) from E. coli"
                     /label=ACP
                     /translation="STIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELVMA
                     LEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     CDS             359..376
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(429..447)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      443..490
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(614..633)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac atatgaaaga aaccgctgct gctaaattcg aacgccagca
       61 catggacagc ccagatctgg gtaccctggt gccacgcggt tccatggctg atatcggatc
      121 cagcactatc gaagaacgcg ttaagaaaat tatcggcgaa cagctgggcg ttaagcagga
      181 agaagttacc aacaatgctt ctttcgttga agacctgggc gcggattctc ttgacaccgt
      241 tgagctggta atggctctgg aagaagagtt tgatactgag attccggacg aagaagctga
      301 gaaaatcacc accgttcagg ctgccattga ttacatcaac ggccaccagg cgctcgagca
      361 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      421 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      481 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
      541 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      601 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      661 ccccgtcaag ctctaaatcg ggggctccct ttagg
//
