LOCUS       sequence-147354-         471 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Plasmid for constitutive expression of gfp-pdt5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pZE27GFP5
FEATURES             Location/Qualifiers
     source          1..471
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(214..300)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
       61 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      121 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      181 ccgcagccga acgccctagg tctagggcgg cggatttgtc ctactcagga gagcgttcac
      241 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
      301 atgcctctag cacgcgtacc atgggatccc ccatcaagct tattactggc gcgctctgtt
      361 cgcctggccc gcgttcagca taaaggtcgg cacttcgttg gtgttttctt cgtttttgtt
      421 cgccgctttg tatagttcat ccatgccatg tgtaatccca gcagctgtta c
//
