LOCUS       sequence-147890-         750 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Mammalian lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Qin H, Hejna M, Liu Y, Percharde M, Wossidlo M, Blouin L, 
            Durruthy-Durruthy J, Wong P, Qi Z, Yu J, Qi LS, Sebastiano V, Song 
            JS, Ramalho-Santos M
  TITLE     YAP Induces Human Naive Pluripotency.
  JOURNAL   Cell Rep. 2016 Mar 15;14(10):2301-12. doi: 
            10.1016/j.celrep.2016.02.036. Epub 2016 Mar 3.
  PUBMED    26947063
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.02.036"; journalName: "Cell Rep"; date: 
            "2016-03-15- 15"; volume: "14"; issue: "10"; pages: "2301-12"
COMMENT     Sequence Label: pGAMA-empty
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(81..87,88..105,106..111))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(136..260)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          276..479
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(299..319)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
       61 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
      121 gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
      181 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
      241 gcttttttgt acaaacttgt gctagctcac gacacctgaa atggaagaaa aaaactttga
      301 accactgtct gaggcttgag aatgaaccaa gatccaaact caaaaagggc aaattccaag
      361 gagaattaca tcaagtgcca agctggccta acttcagtct ccacccactc agtgtgggga
      421 aactccatcg cataaaaccc ctccccccaa cctaaagacg acgtactcca aaagctcgag
      481 aactaatcga ggtgcctgga cggcgcccgg tactccgtgg agtcacatga agcgacggct
      541 gaggacggaa aggccctttt cctttgtgtg ggtgactcac ccgcccgctc tcccgagcgc
      601 cgcgtcctcc attttgagct ccctgcagca gggccgggaa gcggccatct ttccgctcac
      661 gcaactggtg ccgaccgggc cagccttgcc gcccagggcg gggcgataca cggcggcgcg
      721 aggccaggca ccagagcagg ccggccagct
//
