LOCUS       sequence-148021-         933 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for surface expressed EGFP (SFFV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim 
            WA
  TITLE     Engineering Customized Cell Sensing and Response Behaviors Using 
            Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. 
            Epub 2016 Jan 28.
  PUBMED    26830878
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.012"; journalName: "Cell"; date: 
            "2016-02-11- 11"; volume: "164"; issue: "4"; pages: "780-91"
COMMENT     Sequence Label: pHR_EGFPligand
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(64..66,67..69,70..780)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 atggagaccg acaccctctt gctctgggtc ctgctgctgt gggttccggg tagtaccgga
       61 gacatggttt ctaagggtga agaattgttc accggagttg tgcccatcct ggttgagctg
      121 gacggagacg ttaacggaca caagttctca gtgagcggcg aaggagaggg cgacgctacg
      181 tacggtaaac tgacgttgaa gttcatctgt acaacaggaa agctgccagt gccctggcct
      241 acactcgtga ccaccctcac ctacggagtc cagtgcttct cccggtaccc cgaccacatg
      301 aaacagcacg atttcttcaa gtccgcgatg cctgagggct atgttcagga acgcaccata
      361 ttcttcaagg acgacggtaa ctataaaacc agggctgaag ttaagttcga gggggacacg
      421 ttggtaaata gaattgagtt gaaaggaatc gatttcaaag aagacggcaa tattttgggc
      481 cacaagctgg agtacaacta caacagccac aacgtgtata ttatggctga taagcagaag
      541 aacggcatca aggtgaactt caaaatccga cacaatatcg aggatggctc tgtgcaactg
      601 gccgaccatt accagcaaaa cacgcctatc ggcgacggtc cggttttgct gccagataac
      661 cactacctct ccacgcaatc agccctctcc aaggatccta acgaaaaaag agaccatatg
      721 gttctgcttg aattcgtgac ggctgctggg atcaccctgg gaatggatga gctgtataag
      781 aacgccgtgg gccaggatac gcaggaggtg atcgtcgtgc cccacagctt gcccttcaag
      841 gtcgtggtta ttagtgctat cctggcgttg gtggtgctga ctattatctc ccttatcata
      901 cttatcatgc tgtggcaaaa aaaaccccga taa
//
