LOCUS       sequence-148061-         962 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the 
            LRR domain) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, 
            Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, 
            Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, 
            Ulevitch RJ, Beutler B
  TITLE     NLRP3 activation and mitosis are mutually exclusive events 
            coordinated by NEK7, a new inflammasome component.
  JOURNAL   Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 
            Dec 7.
  PUBMED    26642356
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.3333"; 
            journalName: "Nat Immunol"; date: "2016-03"; volume: "17"; issue: 
            "3"; pages: "250-8"
COMMENT     Sequence Label: pcDNA3-N-Flag-NLRP3 711-1034
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    107..331
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     320..337
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        357..375
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     357..374
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
       61 tggggatacc ccctagagcc ccagctggtt ctttccgcct cagaagccat agagcccacc
      121 gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc
      181 ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat
      241 taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa
      301 acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgagc tctagcattt
      361 aggtgacact atagaatagg gccctctaga tgcatgctcg agctaccagg aaatctcgaa
      421 gactatagtc agctcaggct tttcttcctg gagcgcttct aaggcacgtt ttgtttcacg
      481 atttaagtac atttcaccca actgtaggct ctgcaggagg cagccctgct gtttcagcac
      541 ctcacagagg gtcaccacgc acagatcgcc aagatcattg ttgcccaggt tcagcttccg
      601 aaggctgtgg ttgtgggtca gaattgtgga gagattccag cagctgtgtg aggtgaggct
      661 gcagttgtct aattccagca tctgtagttt acagtccggg tgcagaagcc cctcacagag
      721 gagcctgagt cctgtgtctc caagggcatt gcttcgtaga tagaggtgtg tgaagttctg
      781 gttggttttg agcacagagg tcagagctga acaacagatt gaagtaaggc cggaattcac
      841 caaccccagc ttctgcaagt tacactgtgg gtccttcatc ttttcacaca aaacttggac
      901 tcctgagtct cccaaggcat tttctccaat gtacagtctg gtcagagaat ggttggagct
      961 ca
//
